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008			240724s2000 xx o ||||0 eng d
020			_a9780879695859 _q(electronic bk.)
020			_z9780879695859
035			_a(MiAaPQ)EBC3137898
035			_a(Au-PeEL)EBL3137898
035			_a(CaPaEBR)ebr10006728
035			_a(OCoLC)928634304
040			_aMiAaPQ _beng _erda _epn _cMiAaPQ _dMiAaPQ
050		4	_aQL537.D76 -- D765 2000eb
082	0		_a595.77/4
100	1		_aSullivan, William.
245	1	0	_aDrosophila Protocols.
250			_a1st ed.
264		1	_aWoodbury : _bCold Spring Harbor Laboratory Press, _c2000.
264		4	_c©2000.
300			_a1 online resource (716 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
505	0		_aIntro -- Contents -- Preface -- Abbreviations -- Preparation and Analysis of Drosophila Mitotic Chromosomes -- In Situ Hybridization to Somatic Chromosomes -- BrdU Labeling of Chromosomes -- Analysis of Meiosis in Fixed and Live Oocytes by Light Microscopy -- Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster -- Preparation and Analysis of Polytene Chromosomes -- In Situ Hybridization to Polytene Chromosomes -- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining -- Fluorescent Analysis of Drosophila Embryos -- Imaginal Discs -- The Neuromuscular Junction -- Histological Techniques for the Drosophila Eye Part I: Larva and Pupa -- Histological Techniques for the Drosophila Eye Part II: Adult -- Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy -- Electrophysiological Approaches to the Neuromusculature -- Functional Assays of the Peripheral and Central Nervous Systems -- GFP and Other Reporters -- Quantitative Microinjection of Drosophila Embryos -- Targeted Disruption of Gene Function in Drosophila by RNA Interference -- Drosophila Cell Culture and Transformation -- Generating Antibodies against Drosophila Proteins -- Photoactivated Gene Expression for Cell- fate Mapping and Cell Manipulation -- Recovery of DNA Sequences Flanking P- element Insertions: Inverse PCR and Plasmid Rescue -- GAL4- mediated Ectopic Gene Expression in Drosophila -- Functional Cell Ablation -- Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones -- Drosophila Microarrays: From Arrayer Construction to Hybridization -- Using Drosophila Genome Databases -- Preparation and Analysis of High- molecular- weight DNA in Drosophila -- Culturing Large Populations of Drosophila for Protein Biochemistry*.
505	8		_aPreparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis -- Preparation of Membrane Proteins from Drosophila Embryos -- Preparing Cytoplasmic Extracts from Drosophila Embryos -- Immunoblotting of Proteins from Single Drosophila Embryos -- Laboratory Culture of Drosophila -- Preparation of Larval and Adult Cuticles for Light Microscopy -- Exposing Drosophila to Neuroactive Drugs -- 1989 Table of Contents from Drosophila: A Laboratory Manual -- Anatomical Drawings of Drosophila -- Solutions and Reagents -- Cautions -- Suppliers -- Trademarks -- Index.
588			_aDescription based on publisher supplied metadata and other sources.
590			_aElectronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
650		0	_aDrosophila -- Laboratory manuals.
655		4	_aElectronic books.
700	1		_aAshburner, Michael.
700	1		_aHawley, R. Scott.
776	0	8	_iPrint version: _aSullivan, William _tDrosophila Protocols _dWoodbury : Cold Spring Harbor Laboratory Press,c2000 _z9780879695859
797	2		_aProQuest (Firm)
856	4	0	_uhttps://ebookcentral.proquest.com/lib/orpp/detail.action?docID=3137898 _zClick to View
999			_c72233 _d72233