| 000 | 07259nam a22004693i 4500 | ||
|---|---|---|---|
| 001 | EBC5612900 | ||
| 003 | MiAaPQ | ||
| 005 | 20240724113457.0 | ||
| 006 | m o d | | ||
| 007 | cr cnu|||||||| | ||
| 008 | 240724s2018 xx o ||||0 eng d | ||
| 020 |
_a9781119378280 _q(electronic bk.) |
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| 020 | _z9781118361986 | ||
| 035 | _a(MiAaPQ)EBC5612900 | ||
| 035 | _a(Au-PeEL)EBL5612900 | ||
| 035 | _a(OCoLC)1048055848 | ||
| 040 |
_aMiAaPQ _beng _erda _epn _cMiAaPQ _dMiAaPQ |
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| 050 | 4 | _aRM300 .B567 2019 | |
| 100 | 1 | _aKomives, Claire. | |
| 245 | 1 | 0 | _aBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts. |
| 250 | _a1st ed. | ||
| 264 | 1 |
_aNewark : _bJohn Wiley & Sons, Incorporated, _c2018. |
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| 264 | 4 | _c©2019. | |
| 300 | _a1 online resource (290 pages) | ||
| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 490 | 1 | _aWiley Series in Biotechnology and Bioengineering Series | |
| 505 | 0 | _aCover -- Title Page -- Copyright -- Contents -- List of Contributors -- Part I Case Study -- Chapter 1 Bacillus and the Story of Protein Secretion and Production -- 1.1 Bacillus as a Production Host: Introduction and Historical Account -- 1.2 The Building of a Production Strain: Genetic Tools for B. subtilis Manipulation -- 1.2.1 Promoters -- 1.2.2 Vectors for Building a Production Strain -- 1.2.3 B. subtilis Competent Cell Transformation -- 1.2.4 Protoplasts‐Mediated Manipulations -- 1.2.5 Genetics by Electroporation -- 1.3 B. subtilis Secretion System and Heterologous Protein Production -- 1.3.1 Bacillus Fermentation and Recovery of Industrial Enzyme -- 1.3.2 Fermentation Stoichiometry -- 1.3.3 Fermentor Kinetics and Outputs -- 1.3.4 Downstream Processing -- 1.4 Summary -- References -- Chapter 2 New Expression Systems for GPCRs -- 2.1 Introduction -- 2.2 Recombinant GPCR Production - Traditional Approaches for Achieving High‐Level Production -- 2.3 Engineered Expression Systems for GPCR Production -- 2.3.1 Bacteria -- 2.3.2 Yeasts -- 2.3.3 Insect Cells -- 2.3.4 Mammalian Cells -- 2.3.5 Transgenic Animals -- 2.3.6 Cell‐Free Systems -- 2.4 Conclusion -- References -- Chapter 3 Glycosylation -- 3.1 Introduction -- 3.2 Types of Glycosylation -- 3.2.1 N‐linked Glycans -- 3.2.2 O‐linked Glycans -- 3.3 Factors Affecting Glycosylation -- 3.3.1 Nutrient Depletion -- 3.3.2 Fed‐batch Cultures and Supplements -- 3.3.3 Specific Culture Supplements -- 3.3.4 Ammonia -- 3.3.5 pH -- 3.3.6 Oxygen -- 3.3.7 Host Cell Systems -- 3.3.8 Other Factors -- 3.4 Modification of Glycosylation -- 3.4.1 siRNA and Gene Knockout/Knockin -- 3.4.2 Glycoprotein Processing Inhibitors and In Vitro Modification of Glycans -- 3.5 Glycosylation Analysis -- 3.5.1 Release of Glycans from Glycoproteins -- 3.5.2 Derivatization of Glycans -- 3.6 Methods of Analysis -- 3.6.1 Lectin Arrays. | |
| 505 | 8 | _a3.6.2 Liquid Chromatography -- 3.6.2.1 HILIC Analysis -- 3.6.2.2 Reversed Phase (RP) and Porous Graphitic Carbon (PGC) Chromatography -- 3.6.2.3 Weak Anion Exchange (WAX) HPLC Analysis -- 3.6.2.4 High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC‐PAD) -- 3.6.3 Capillary Electrophoresis (CE) -- 3.6.4 Fluorophore‐assisted Carbohydrate Electrophoresis (FACE) and CGE‐LIF -- 3.6.5 Mass Spectrometry (MS) -- 3.6.5.1 Ionization -- 3.6.5.2 Derivatization Techniques Used for MS Analysis of Glycans -- 3.6.5.3 Fragmentation of Carbohydrates -- 3.7 Conclusion -- References -- Part II Bioreactors -- Chapter 4 Bioreactors for Stem Cell and Mammalian Cell Cultivation -- 4.1 Overview of (Mammalian and Stem) Cell Culture Engineering -- 4.1.1 Cell Products for Therapeutics -- 4.1.2 Cell as a Product: Stem Cells -- 4.2 Bioprocess Characterization -- 4.2.1 Cell Cultivation Methods -- 4.2.2 Cell Metabolism -- 4.2.3 Culture Medium Design -- 4.2.4 Culture Parameters -- 4.2.5 Culture Modes -- 4.3 Cell Culture Systems -- 4.3.1 Static Culture Systems -- 4.3.2 Roller Bottles -- 4.3.3 Spinner Flask -- 4.3.4 Airlift Bioreactor -- 4.3.5 Fixed/Fluidized‐Bed Bioreactor -- 4.3.6 Wave Bioreactor -- 4.3.7 Rotating‐Wall Vessel Bioreactor -- 4.3.8 Stirred Tank Bioreactor -- 4.3.8.1 Agitation/Shear Stress -- 4.4 Cell Culture Modeling -- 4.5 Case Studies -- 4.5.1 Antibody Production in Bioreactor Systems -- 4.5.2 mESC Expansion on Microcarriers in a Stirred Tank Bioreactor -- 4.6 Concluding Remarks -- List of Symbols -- References -- Chapter 5 Model‐Based Technologies Enabling Optimal Bioreactor Performance -- 5.1 Introduction -- 5.2 Basics -- 5.2.1 Balances -- 5.2.2 Model Identification -- 5.2.3 Model‐Based Process Optimization -- 5.3 Examples -- 5.3.1 Model‐Based State Estimation -- 5.3.1.1 Static Model Approach -- 5.3.1.2 Dynamic Alternatives. | |
| 505 | 8 | _a5.3.2 Optimizing Open Loop‐Controlled Cultivations -- 5.3.2.1 Robust Cultivation Profiles -- 5.3.2.2 Evolutionary Modeling Approach -- 5.3.3 Optimization by Model‐Aided Feedback Control -- 5.3.3.1 Improving the Basic Control -- 5.3.3.2 Optimizing the Amount of Soluble Product -- 5.3.4 CO2‐Removal in Large‐Scale Cell Cultures -- 5.4 Conclusion -- References -- Chapter 6 Monitoring and Control of Bioreactor: Basic Concepts and Recent Advances -- 6.1 Introduction -- 6.2 Challenges in Bioprocess Control -- 6.2.1 Process Dynamics and Modeling -- 6.2.2 Limits of Hardware and Software and Their Integration -- 6.2.3 Regulatory Aspects -- 6.3 Basic Elements of Bioprocess Control -- 6.3.1 Bioprocess Monitoring -- 6.3.2 Parameter Estimators -- 6.3.3 Bioprocess Modeling -- 6.4 Current Practices in Bioprocess Control -- 6.4.1 PID Control -- 6.4.2 Model‐Based Control -- 6.4.3 Adaptive Control -- 6.4.4 Nonlinear Control -- 6.5 Intelligent Control Systems -- 6.5.1 Fuzzy Control -- 6.5.2 Neural Control -- 6.5.3 Statistical Process Control -- 6.5.4 Integrated and Plant‐Wide Bioprocess Control -- 6.5.5 Metabolic Control -- 6.6 Summary -- 6.7 Future Perspectives -- Acknowledgments -- References -- Part III Host Strain Technologies -- Chapter 7 Metabolic Engineering for Biocatalyst Robustness to Organic Inhibitors -- 7.1 Introduction -- 7.2 Mechanisms of Inhibition -- 7.3 Mechanisms of Tolerance -- 7.4 Membrane Engineering -- 7.5 Evolutionary and Metagenomic Strategies for Increasing Tolerance -- 7.6 Reverse Engineering of Improved Strains -- 7.7 Concluding Remarks -- Acknowledgments -- References -- Index -- EULA. | |
| 588 | _aDescription based on publisher supplied metadata and other sources. | ||
| 590 | _aElectronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. | ||
| 650 | 0 | _aPharmaceutical technology. | |
| 650 | 0 | _aBioreactors. | |
| 655 | 4 | _aElectronic books. | |
| 700 | 1 | _aZhou, Weichang. | |
| 776 | 0 | 8 |
_iPrint version: _aKomives, Claire _tBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts _dNewark : John Wiley & Sons, Incorporated,c2018 _z9781118361986 |
| 797 | 2 | _aProQuest (Firm) | |
| 830 | 0 | _aWiley Series in Biotechnology and Bioengineering Series | |
| 856 | 4 | 0 |
_uhttps://ebookcentral.proquest.com/lib/orpp/detail.action?docID=5612900 _zClick to View |
| 999 |
_c7029 _d7029 |
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