| 000 | 08756nam a22005053i 4500 | ||
|---|---|---|---|
| 001 | EBC31253159 | ||
| 003 | MiAaPQ | ||
| 005 | 20240724115947.0 | ||
| 006 | m o d | | ||
| 007 | cr cnu|||||||| | ||
| 008 | 240724s2021 xx o ||||0 eng d | ||
| 020 |
_a9780750320801 _q(electronic bk.) |
||
| 020 | _z9780750320818 | ||
| 035 | _a(MiAaPQ)EBC31253159 | ||
| 035 | _a(Au-PeEL)EBL31253159 | ||
| 035 | _a(OCoLC)1429737102 | ||
| 040 |
_aMiAaPQ _beng _erda _epn _cMiAaPQ _dMiAaPQ |
||
| 050 | 4 | _aQD96.F56 W645 2020 | |
| 082 | 0 | _a543.56 | |
| 100 | 1 | _aWohland, Thorsten. | |
| 245 | 1 | 3 | _aAn Introduction to Fluorescence Correlation Spectroscopy. |
| 250 | _a1st ed. | ||
| 264 | 1 |
_aBristol : _bInstitute of Physics Publishing, _c2021. |
|
| 264 | 4 | _c©2020. | |
| 300 | _a1 online resource (368 pages) | ||
| 336 |
_atext _btxt _2rdacontent |
||
| 337 |
_acomputer _bc _2rdamedia |
||
| 338 |
_aonline resource _bcr _2rdacarrier |
||
| 490 | 1 | _aBiophysical Society-IOP Series | |
| 505 | 0 | _aIntro -- Preface -- Acknowledgements -- About the authors -- Thorsten Wohland -- Sudipta Maiti -- Radek Macháň -- Chapter 1 Introduction -- 1.1 What is fluorescence correlation spectroscopy all about? -- 1.2 What do 'fluorescence', 'correlation' and 'spectroscopy' have to do with measuring change? -- 1.3 What can FCS do for you? -- 1.4 What does an FCS measurement involve? -- 1.5 A brief history of FCS -- 1.5.1 Early work -- 1.5.2 The year of the invention -- 1.5.3 The initial progress -- 1.6 Critical technical steps of the revolution -- 1.6.1 Fluorescence: towards single molecule sensitivity -- 1.6.2 Microscopic volume -- 1.6.3 The confocal technique -- 1.6.4 Modern detectors -- 1.6.5 The data processors -- 1.7 Where is FCS now? -- References -- Chapter 2 Correlation functions -- 2.1 Introduction -- 2.2 Fluctuations -- 2.3 Correlations -- 2.4 From correlation coefficient to correlation function -- 2.5 The autocorrelation function (ACF) and its properties -- 2.6 The cross-correlation function (CCF) and its properties -- 2.7 Fluctuations and correlations -- 2.8 Synopsis -- 2.9 Exercises -- References -- Chapter 3 Fluorescence excitation and detection -- 3.1 The probe volume in FCS -- 3.1.1 Introduction -- 3.1.2 The significance of the size of the probe volume -- 3.1.3 A brief introduction to the generation of the fluorescence signal -- 3.1.4 Optical designs applied to obtain an appropriate probe volume for FCS -- 3.2 Photon detection -- 3.2.1 Photon counting -- 3.2.2 Array detectors -- 3.2.3 Photomultiplier tubes -- 3.3 Exercises -- References -- Chapter 4 Data structure, correlation and processing -- 4.1 Software correlators -- 4.1.1 Binned intensity trace-linear correlator -- 4.1.2 Binned intensity trace-multiple-tau correlator -- 4.1.3 Time-tagged intensity trace -- 4.1.4 Cross-correlation calculation and correlation function amplitude. | |
| 505 | 8 | _a4.1.5 Correlation function calculation via Fourier transform -- 4.2 Hardware correlators and their comparison with software correlators -- 4.3 Temporal resolution of correlation functions -- 4.4 Statistical filtering in correlation function calculation -- 4.4.1 Fluorescence lifetime and its integration into FCS datasets -- 4.4.2 Generation of statistical filters in fluorescence lifetime correlation spectroscopy (FLCS) -- 4.4.3 Generalisation of the FLCS principle -fluorescence spectral correlation spectroscopy (FSCS) and filtered FCS (fFCS) -- 4.5 Synopsis -- 4.6 Exercises -- References -- Chapter 5 Theoretical FCS models -- 5.1 The autocorrelation function for diffusion -- 5.2 General characteristics of the ACF for diffusion -- 5.3 Including multiple particles -- 5.4 Anomalous diffusion -- 5.5 Flow -- 5.6 Including multiple processes -- 5.7 Spatial and spatiotemporal correlation techniques -- 5.7.1 Two-focus FCS -- 5.7.2 Scanning FCS -- 5.7.3 Circular scanning fluorescence correlation spectroscopy -- 5.7.4 Image correlation spectroscopy (ICS) -- 5.7.5 Spatiotemporal image correlation spectroscopy (STICS) -- 5.7.6 Raster image correlation spectroscopy (RICS) -- 5.7.7 Imaging fluorescence correlation spectroscopy (Imaging FCS) -- 5.7.8 The FCS diffusion law -- 5.8 Other FCS modalities -- 5.9 Synopsis -- 5.10 Exercises -- References -- Chapter 6 Theoretical fluorescence cross-correlation spectroscopy (FCCS) models -- 6.1 Introduction -- 6.2 Dual-colour FCCS (DC-FCCS) -- 6.2.1 Cross-correlation amount -- 6.2.2 Unequal and non-aligned observation volumes -- 6.2.3 Spectral crosstalk -- 6.2.4 Non-correlated background -- 6.2.5 Non-fluorescent binding partners and free fluorophores -- 6.2.6 Fluorescence quenching and Förster resonance energy transfer (FRET) between fluorophores a and b -- 6.2.7 Complex stoichiometry. | |
| 505 | 8 | _a6.3 FCCS modalities derived from DC-FCCS -- 6.3.1 Single-wavelength FCCS (sw-FCCS) -- 6.3.2 FCCS with alternating laser excitation -- 6.4 Statistical filtering in FCCS -- 6.4.1 Statistical filtering and sources of DC-FCCS artefacts -- 6.4.2 Statistical filtering and negative CCF amplitudes -- 6.4.3 Quasi pulsed interleaved excitation FCCS (PIE-FCCS) -- 6.4.4 Reaction kinetics studied by FCCS -- 6.5 Synopsis -- 6.6 Exercises -- References -- Chapter 7 Artefacts in FCS -- 7.1 Background -- 7.2 Rare events -- 7.3 Bleaching -- 7.4 Sample movement -- 7.5 Detector-related artefacts: after-pulsing and dead time -- 7.6 Detector saturation -- 7.7 Fluorophore saturation -- 7.8 Scattering -- 7.9 Autofluorescence -- 7.10 Sample topology -- 7.11 Immobile particles -- 7.12 Refractive index mismatch -- 7.13 Exercises -- References -- Chapter 8 Data fitting -- 8.1 Introduction -- 8.2 What do we minimize? -- 8.3 The data structure and bias in FCS -- 8.3.1 The data structure in FCS -- 8.3.2 The bias of correlation functions -- 8.4 The standard deviation in FCS -- 8.4.1 Koppel's standard deviation and its modifications -- 8.4.2 Standard deviations from multiple measurements -- 8.4.3 Standard deviation derived from the intensity trace -- 8.4.4 Standard deviation and bias within the ACF -- 8.4.5 The problem of correlations within the ACF -- 8.5 Non-linear least squares fit -- 8.5.1 Least squares and the χ2 function -- 8.5.2 The Levenberg-Marquardt fitting algorithm -- 8.6 Generalized least squares fit -- 8.6.1 The covariance matrix for the ACF -- 8.6.2 Regularization -- 8.7 Global fit -- 8.8 Maximum entropy method -- 8.9 Pairwise model selection using the F-test -- 8.10 Bayes model selection -- 8.11 Practical aspects -- 8.12 Synopsis -- 8.13 Exercises -- References -- Chapter 9 FCS and FCCS measurement strategies -- 9.1 Measuring concentrations by FCS. | |
| 505 | 8 | _a9.1.1 Concentration range accessible by FCS -- 9.1.2 Effective observation volume calibration -- 9.1.3 Molecular brightness determined by FCS -- 9.2 Characterising molecular diffusion by FCS -- 9.2.1 Confocal observation volume radius calibration -- 9.2.2 Calibration-free FCS modalities -- 9.2.3 Imaging FCS and its calibration -- 9.2.4 Separation of multiple processes contributing to the ACF temporal decay -- 9.2.5 Diffusion in two-dimensional systems studied by FCS -- 9.3 Molecular interactions studies by FCS -- 9.3.1 Using changes in the ACF amplitude -- 9.3.2 Using changes in the ACF temporal decay -- 9.4 Molecular interactions studies by FCCS -- 9.4.1 The importance of relating the CCF amplitude to ACF amplitudes -- 9.4.2 Determination of the FCCS experiment dynamic range -- 9.5 Synopsis -- 9.6 Exercises -- References -- Chapter 10 Where to go from here? -- 10.1 Introduction -- 10.2 What FCS can and cannot do -- 10.3 Data acquisition -- 10.4 Data analysis -- 10.5 Related techniques -- 10.6 Some final remarks -- References -- Chapter -- Chapter 2 -- Chapter 3 -- Chapter 4 -- Chapter 5 -- Chapter 6 -- Chapter 7 -- Chapter 8 -- Chapter 9. | |
| 520 | _aA comprehensive course on fluorescence correlation spectroscopy covering an explanation of basic concepts, derivation of theoretical models, and experimental realisation of the technique. | ||
| 588 | _aDescription based on publisher supplied metadata and other sources. | ||
| 590 | _aElectronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. | ||
| 650 | 0 | _aFluorescence spectroscopy. | |
| 655 | 4 | _aElectronic books. | |
| 700 | 1 | _aMaiti, Sudipta. | |
| 700 | 1 | _aMacháň, Radek. | |
| 776 | 0 | 8 |
_iPrint version: _aWohland, Thorsten _tAn Introduction to Fluorescence Correlation Spectroscopy _dBristol : Institute of Physics Publishing,c2021 _z9780750320818 |
| 797 | 2 | _aProQuest (Firm) | |
| 830 | 0 | _aBiophysical Society-IOP Series | |
| 856 | 4 | 0 |
_uhttps://ebookcentral.proquest.com/lib/orpp/detail.action?docID=31253159 _zClick to View |
| 999 |
_c36811 _d36811 |
||