000			03899nam a22004453i 4500
001			EBC5897808
003			MiAaPQ
005			20240724113940.0
006			m o d |
007			cr cnu||||||||
008			240724s2013 xx o ||||0 eng d
020			_a9781908230874 _q(electronic bk.)
020			_z9781908230225
035			_a(MiAaPQ)EBC5897808
035			_a(Au-PeEL)EBL5897808
035			_a(OCoLC)1048368651
040			_aMiAaPQ _beng _erda _epn _cMiAaPQ _dMiAaPQ
050		4	_aQP606.D46 .R43 2013
082	0		_a574.873282
100	1		_aSaunders, Nick A.
245	1	0	_aReal-Time PCR : _bAdvanced Technologies and Applications.
250			_a1st ed.
264		1	_aNorfolk : _bCaister Academic Press, _c2013.
264		4	_c©2013.
300			_a1 online resource (289 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
505	0		_aIntro -- Contents -- Contributors -- Preface -- Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR -- Ch 02: Internal and Other Controls for Real-time PCR Validation -- Ch 03: Analysis of mRNA Expression by Real-time PCR -- Ch 04: Detection of Biothreats by Real-time PCR -- Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents -- Ch 06: Applications in Clinical Microbiology -- Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR -- Ch 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCR -- Ch 09: Real-time PCR Arrays -- Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases -- Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay -- Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation,Verification and Implementation -- Index.
520			_aThis essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods and practice of real-time PCR. The initial chapters cover the important aspects of real-time PCR including choosing an instrument and probe system, set-up, nucleic acid synthesis, sample extraction controls, and validation and data analysis. Further chapters provide a comprehensive overview of important real-time PCR methodologies such as quantification, expression analysis and mutation detection. This is complemented by the final chapters, which address the application of real-time PCR to diagnosis of infectious diseases, biodefence, veterinary science, food authenticity and molecular haplotyping. This timely and authoritative volume serves both as a basic introduction to real-time PCR and as a source of current trends and applications for those already familiar with the technology. The editors also aim to stimulate readers of all levels to develop their own innovative approaches to real-time PCR. An essential book for all laboratories using PCR.
588			_aDescription based on publisher supplied metadata and other sources.
590			_aElectronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
650		0	_aPolymerase chain reaction.
650		0	_aMolecular biology-Technique.
655		4	_aElectronic books.
700	1		_aLee, Martin A.
776	0	8	_iPrint version: _aSaunders, Nick A. _tReal-Time PCR _dNorfolk : Caister Academic Press,c2013 _z9781908230225
797	2		_aProQuest (Firm)
856	4	0	_uhttps://ebookcentral.proquest.com/lib/orpp/detail.action?docID=5897808 _zClick to View
999			_c13418 _d13418