Clinical Virology Manual.

Cover -- Half-Title Page -- Title Page -- Copyright Page -- DEDICATION -- Contents -- Contributors -- Editor Biographies -- Preface to the Fifth Edition -- SECTION I: General Topics in Clinical Virology -- 1. The Taxonomy, Classification, and Characterization of Medically Important Viruses -- WHAT ARE VIRUSES? -- Papillomaviruses -- Picornaviruses -- Adenoviruses -- Norovirus -- REFERENCES -- 2. Quality Assurance and Quality Control in Clinical and Molecular Virology -- REGULATORY REQUIREMENTS -- VIROLOGY QUALITY ASSURANCE -- CLINICAL LABORATORY PERSONNEL -- PROCEDURE MANUAL -- SPECIMEN COLLECTION AND TRANSPORT -- REAGENTS, MATERIALS, AND CELL CULTURES -- MOLECULAR TESTING -- VIRAL SEROLOGY AND DIRECT ANTIGEN DETECTION -- INSTRUMENTATION -- QUALITY CONTROL AND STANDARDS -- PROFICIENCY TESTING -- CORRECTIVE AND PREVENTIVE ACTION (CAPA) -- TEST VERIFICATION AND VALIDATION -- REFERENCES -- 3. Regulatory Compliance -- REFERENCES -- 4. Laboratory Safety -- SAFETY IN THE CLINICAL VIROLOGY LABORATORY -- Principles of Biosafety -- A Culture of Safety -- Disinfection and Sterilization -- Chemical Safety -- Electrical Safety -- Fire Safety -- Biosafety -- Recommended Work Practices -- Safe Transport of Specimens/Biological Agents -- Waste Disposal -- Biosecurity -- Summary -- REFERENCES -- 5. Laboratory Design -- THE PHASES OF LABORATORY DESIGN -- Planning -- Design -- Bidding -- Construction -- Occupancy -- IMPORTANT ELEMENTS OF LABORATORY DESIGN FOR CLINICAL VIROLOGY -- WORKFLOW -- SUMMARY -- REFERENCES -- SECTION II: Laboratory Procedures for Detecting Viruses -- 6. Specimen Requirements Selection, Collection, Transport, and Processing -- IMPORTANCE OF TEST SELECTION, SPECIMEN SELECTION, AND SPECIMEN COLLECTION -- HOST FACTORS THAT AFFECT SPECIMEN COLLECTION -- VIRAL FACTORS THAT AFFECT SPECIMEN COLLECTION.

IMPORTANCE OF TEST PLATFORM FOR SPECIMEN COLLECTION -- SPECIMEN TYPE -- SPECIMEN COLLECTION -- REPEAT SPECIMEN COLLECTION -- SPECIMEN PROCESSING -- SPECIMEN TRANSPORT AND STORAGE -- FUTURE CONSIDERATIONS -- REFERENCES -- 7. Primary Isolation of Viruses -- VIRUS ISOLATION IN CELL CULTURES -- Types of Cell Culture -- Variation in Sensitivity to Different Viruses -- Supplies and Equipment Needed -- Obtaining and Processing Specimens -- Conventional Cell Culture -- Inoculation and Incubation -- Detection of Virus-Induced Effects -- Identification of Virus Isolates -- RAPID CULTURE METHODS -- Centrifugation Culture (Shell Vial Technique) -- Mixed (Co-Cultivated) Cell Cultures and Monoclonal Antibody Pools -- Genetically Modified Cell Lines -- VIRUSES COMMONLY ISOLATED IN CONVENTIONAL CELL CULTURE -- Herpes Simplex Viruses (HSV-1 and HSV-2) -- Varicella Zoster Virus (VZV) -- Cytomegalovirus (CMV) -- Adenovirus -- Enterovirus -- Rhinovirus -- Influenza -- Parainfluenza (PIV) -- Respiratory Syncytial Virus (RSV) -- QUALITY ASSURANCE AND TROUBLESHOOTING -- APPLICATIONS IN DIAGNOSTIC VIROLOGY -- REFERENCES -- 8. Viral Antigen Detection -- BASIC CONCEPTS OF VIRAL ANTIGEN DETECTION METHODS -- Immunofluorescence (FA) -- Immunochromatography (Lateral Flow) -- Enzyme Immunoassay (EIA) -- APPLICATION OF VIRAL ANTIGEN DETECTION IN DIAGNOSTIC VIROLOGY -- QUALITY ASSURANCE AND REPORTING -- CONCLUSIONS -- REFERENCES -- 9. Serologic (Antibody Detection) Methods -- NEUTRALIZATION -- Overview -- Methods -- Applications -- Quality Assessment and Troubleshooting -- HEMAGGLUTINATION INHIBITION -- Overview -- Methods -- Applications -- Quality Assessment, Troubleshooting and Limitations -- IMMUNOFLUORESCENCE ASSAYS -- Overview -- Methods -- Applications -- Quality Assessment, Troubleshooting, and Limitations -- ENZYME IMMUNOASSAY -- Overview -- Methods -- Applications.

Quality Assessment and Troubleshooting -- IMMUNOGLOBULIN M DETERMINATIONS -- Overview -- Methods -- Applications -- Quality Assessment and Troubleshooting -- WESTERN BLOT -- Overview -- Methods -- Quality Assessment and Limitations -- Applications -- SUMMARY -- REFERENCES -- 10. Nucleic Acid Extraction in Diagnostic Virology -- MANUAL AND SEMI-AUTOMATED METHODS FOR NA EXTRACTION IN DIAGNOSTIC VIROLOGY -- Phenol-Chloroform -- Guanidinium Thiocyanate-Phenol-Chloroform Extraction -- QIAamp Viral RNA Mini Kit -- QIAamp DNA Mini and Blood Mini Kit -- NucliSens miniMAG System -- TruTip Technology -- AUTOMATED METHODS FOR NA EXTRACTION IN DIAGNOSTIC VIROLOGY -- Automation of Manual Processes -- Automation of NA Extraction Using Magnetic Particle Capture and Robotics -- Automation of Extraction Plus Setup of Master and Reaction Mix -- Systems with Full Sample In-Answer Out Automation -- Performance of the Automated Systems -- Literature References Comparing Performance of Extraction Systems -- Emerging Extraction Technology -- Validation of NA Extraction Systems as Part of a Clinical Laboratory Viral Molecular Diagnostics Assay -- REFERENCES -- 11. Nucleic Acid Amplification by Polymerase Chain Reaction -- CONVENTIONAL PCR -- NESTED PCR -- REAL-TIME DETECTION OF NUCLEIC ACIDS BY PCR -- Multiplex PCR -- EMERGING TECHNIQUES -- PCR-ESI/MALDI -- Digital PCR (dPCR) -- CONCLUSIONS -- REFERENCES -- 12. Isothermal Nucleic Acid Amplification Methods -- TRANSCRIPTION-BASED AMPLIFICATION METHODS: NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (NASBA) AND TRANSCRIPTION MEDIATED AMPLIFICATION (TMA) -- Application of NASBA in the Clinical Virology Laboratory -- Application of TMA in the Clinical Virology Laboratory -- STRAND DISPLACEMENT AMPLIFICATION (SDA) -- Application of SDA in the Clinical Virology Laboratory -- LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) AND Q-LAMP.

Application of LAMP/Q-LAMP in the Clinical Virology Laboratory -- NICKING ENDONUCLEASE AMPLIFICATION REACTION (NEAR) -- Application of NEAR in the Clinical Virology Laboratory -- RECOMBINASE POLYMERASE AMPLIFICATION (RPA) -- Application of RPA in the Clinical Virology Laboratory -- PRESENT STATE AND FUTURE ASPECTS OF’DIFFERENT ISOTHERMAL NUCLEIC ACID AMPLIFICATION METHODS USEFUL FOR THE’CLINICAL VIROLOGY LABORATORY -- Advantages and Disadvantages of Transcription-Based Amplification Methods (NASBA and TMA) -- Advantages and Disadvantages of SDA -- Advantages and Disadvantages of LAMP and Q-LAMP -- Advantages and Disadvantages of NEAR -- Advantages and Disadvantages of RPA -- CONCLUSION AND FUTURE PERSPECTIVES -- REFERENCES -- 13. Quantitative Molecular Methods -- COMMON QUANTITATIVE METHODS -- PCR -- ASSESSMENT OF QUANTITATIVE ASSAY PERFORMANCE AND LIMITATIONS -- General Concepts -- APPLICATIONS OF VIRAL LOADS -- Human Immunodeficiency Virus 1 (HIV-1) -- Hepatitis C Virus (HCV) -- Hepatitis B Virus (HBV) -- Cytomegalovirus (CMV) -- Epstein-Barr Virus -- Other Herpesviruses -- BK virus (BKV) -- SUMMARY AND FUTURE IMPLICATIONS -- REFERENCES -- 14. Signal Amplification Methods -- HYBRID CAPTURE TECHNOLOGY -- Principles and Characteristics of HC -- Application of HC in the Clinical Virology Laboratory -- BRANCHED DNA (BDNA) TECHNOLOGY -- Principles and Characteristics of bDNA -- Application of bDNA in the Clinical Virology Laboratory -- SUMMARY -- REFERENCES -- 15. DNA Sequencing for Clinical and Public Health Virology: Some Assembly Required -- DNA SEQUENCING TECHNOLOGIES -- Sanger Sequencing -- Pyrosequencing -- Next Generation Sequencing -- Bioinformatics -- Applications of Sequencing to Virology -- Viral Population Analysis -- CONCLUSIONS -- REFERENCES -- 16. Phenotypic and Genotypic Antiviral Susceptibility Testing.

ANTIVIRAL SUSCEPTIBILITY TESTING -- Culture-Based Systems -- PCR-Based Methods -- Sequencing Methods -- ANTIVIRAL TREATMENT AND SUSCEPTIBILITY TESTING FOR SELECT VIRUSES -- Herpes Viruses -- Influenza Viruses -- Hepatitis Viruses -- HIV -- Discussion -- REFERENCES -- 17. Point-of-Care Diagnostic Virology -- ANTIGEN DETECTION ASSAYS: RESPIRATORY VIRUSES -- Influenza Virus -- Respiratory Syncytial Virus -- ANTIGEN DETECTION ASSAYS: GASTROINTESTINAL VIRUSES -- Rotavirus -- Norovirus -- ANTIBODY DETECTION ASSAYS -- Hepatitis C Virus -- Epstein-Barr Virus -- Human Immunodeficiency Virus -- NUCLEIC ACID DETECTION ASSAYS -- Enteroviruses -- Respiratory Viruses -- Gastrointestinal Viruses -- QUALITY MANAGEMENT -- SUMMARY -- REFERENCES -- 18. Future Technology -- APPLICATIONS IN DIAGNOSTIC VIROLOGY -- Next-Generation Sequencing -- Digital Polymerase Chain Reaction -- Polymerase Chain Reaction/Electrospray Ionization Mass Spectrometry -- Viral Microarray-Based Assays -- Surface Plasmon Resonance -- Digital Immunoassays for Rapid Detection and Differentiation of Influenza Viruses -- Sofia Influenza A+B Fluorescent Immunoassay -- BD Veritor System Flu A+B Immunoassay -- Emerging Point-of-Care Diagnostics -- Influenza Viruses -- Human Immunodeficiency Virus -- Ebola Virus -- FilmArray BioThreat Panel -- Summary -- REFERENCES -- SECTION III: Viral Pathogens -- 19. Respiratory Viruses -- CLASSIFICATION AND BIOLOGY -- Influenza Virus -- RSV and HMPV -- Parainfluenza Virus -- Rhinovirus -- Coronavirus -- Adenovirus -- EPIDEMIOLOGY -- Influenza Virus -- RSV and HMPV -- Parainfluenza Virus -- Rhinovirus -- Coronavirus -- Adenovirus -- CLINICAL SIGNIFICANCE -- Influenza Virus -- RSV and HMPV -- Parainfluenza Virus -- Rhinovirus -- Coronavirus -- Adenovirus -- TREATMENT AND PREVENTION -- Influenza Virus -- RSV and HMPV -- Parainfluenza Virus -- Rhinovirus -- Coronavirus.

Adenovirus.

Description based on publisher supplied metadata and other sources.

Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.