Flow Cytometry in Microbiology : Technology and Applications.

Intro -- Contents -- Current Books of Interest -- Contributors -- Introduction -- 1: Microbial Cytometry: What It Was, Is, and May Be -- Introduction -- Tasks in microbial cytometry: motives and methods -- From microscopy to cytometry: more than meets the eye -- Intrinsic parameters -- Extrinsic parameters -- Microscopy versus cytometry: the eyes do not have it -- Cytometric options: thinking outside and inside the box -- 2: Non-destructive On-chip Imaging Cytometry Assay for Constructive On-chip Cellomics Studies -- Introduction -- A fully automated on-chip imaging flow cytometry system -- Applications of on-chip imaging cytometry technologies -- Summary -- 3: Application of Flow Cytometry to Environmental Biotechnology -- Introduction -- Application to drinking water quality -- Application to wastewater treatments -- Application to solid waste treatments -- Bacterial viability in soils and sediments -- Future trends -- 4: Flow Cytometry and Microbial Community Fingerprinting -- What are natural communities? -- Community structure analysis using flow cytometry -- Community composition analysis using fingerprinting techniques -- Combination of community fingerprinting and single cell analysis by cell sorting -- 5: Application of Flow Cytometry to the Detection of Pathogenic Bacteria -- Microbial pathogenesis -- Advantages of flow cytometry for pathogen detection -- Challenges in pathogen detection -- Instrument set up -- Sample preparation -- Immunolabelling -- Fluorescence in situ hybridisation (FISH) -- Green fluorescent protein (GFP) -- Quantum dot labelling -- FCM analysis of food samples -- FCM analysis of clinical samples -- Physiology of microbial pathogens and FCM analysis -- Cell separation/cell sorting -- Conclusion -- 6: The Use of Flow Cytometry to Study Sporeforming Bacteria -- Introduction -- The importance of spore formers.

Spore formers and flow cytometry: a review of past studies -- Potential applications of flow cytometry to the study of spore formers -- Conclusions -- 7: Flow Cytometry of Yeasts and Other Fungi -- Introduction -- Flow cytometry and FACS to investigate cell and molecular biology -- Medical applications of FCM -- FCM for bioprocess monitoring and development -- Cell engineering and screening using FACS -- Molecular screening methods using yeast and FACS -- New methods and technology related to, and developed from, FCM and FACS -- Conclusion -- 8: The Application of Flow Cytometry to the Study of Lactic Acid Bacteria Fermentations -- Introduction -- Traditional methods used to monitor LAB and a comparison with flow cytometry methodology -- Flow cytometry (FCM) -- Specific examples of flow cytometry and LAB analysis -- Starter performance -- Cheese manufacture and FCM applications -- Probiotic bacteria -- Sample preparation -- Conclusion -- 9: Flow Cytometry for Rapid Microbiological Analysis of Drinking Water: From Science to Practice - An Unfinished Story -- Setting the scene -- The 'method dilemma' in microbiological water analysis -- Developing FCM-based methods in the sheltered lab with an eye on application -- Subjecting practitioners to rapid cell counting (a personal account) -- Basics - counting cells, big and small -- More FCM-based methods with potential for routine monitoring -- Examples selected from practice -- Outlook and some final comments -- Index.

In this text renowned authors have brought together a wealth of experience demonstrating the power and limitations of flow cytometry as it currently stands in the field of microbiology. The book commences with an overview of flow cytometry from Professor Howard Shapiro one of the most eminent scientists in the area of flow cytometry. Further chapters discuss cytometry technology and applications in environmental biotechnology, microbial community fingerprinting, clinical microbiology, lactic acid bacteria, spore forming bacteria, yeasts and fungi, water analysis and chip-based cytometry assays. The final chapter describes the adoption of flow cytometry to routine water quality analysis in Switzerland and provides a fascinating case study of how this emerging microbial technology overcame technical, regulatory and practical issues to become a standard rapid quality control methodology. The book provides a thorough description of flow cytometry and includes practical and up-to-date information aimed specifically at microbiologists. A major work on flow ctyometry for microbiologists, this book should be available in all microbiology libraries.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.