MALDI-TOF Mass Spectrometry in Microbiology.

Intro -- Contents -- Contributors -- Current Books of Interest -- Preface -- Introduction -- A personal vision of the MALDI-TOF-MS journey from obscurity to frontline diagnostics -- Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry for the Clinical Laboratory -- Introduction -- Preanalytical -- Analytical -- Postanalytical -- Postanalytical -- Summary -- 2. Analysis of Anaerobes and Some Other Fastidious Bacteria -- Introduction -- Early developments of databases for anaerobes -- Performance of the mass spectrometer-based identification of clinically relevant anaerobes in routine laboratories -- Pre-analytical requirements for species level identification of anaerobic bacteria -- Use of MALDI-TOF MS for the typing of anaerobic bacteria -- Direct identification of anaerobic bacteria from positive blood cultures -- Use of MALDI-TOF MS for antibiotic resistance determination of anaerobic bacteria -- Use of MALDI-TOF MS to identify other fastidious bacteria -- To summarise the benefits of MALDI-TOF MS in clinical microbiology laboratory practice dealing with fastidious bacteria such as anaerobes and members of the HACEK group -- 3. Identification, Typing and Susceptibility Testing of Fungi (Including Yeasts) by MALDI-TOF MS -- Introduction -- MALDI-TOF MS systems in use -- MALDI-TOF MS identification of yeast isolates -- Analysis of filamentous fungi -- Susceptibility testing of yeasts and filamentous fungi -- Quality control -- 4. Molecular Typing of Bacteria/Fungi Using MALDI-TOF MS -- Introduction -- MALDI-TOF-MS technique -- Use of MALDI-typing for Gram-positive bacteria -- Use of MALDI-typing for Gram-negative bacteria -- Use of MALDI-typing for fungi -- Time- and cost-effectiveness -- Limitations of MALDI-typing -- Concluding remarks -- 5. MALDI-TOF MS for Determination of Resistance to Antibiotics.

Antibiotic resistance -- Need for detection of antibiotic resistance and its mechanisms -- Categorization of clinical isolates based on their clinical breakpoints -- Determination of resistance mechanisms -- Conclusions and future perspectives -- 6. Application of MALDI-TOF MS in Veterinary and Food Microbiology -- Introduction -- Veterinary microbiology -- Food microbiology -- Conclusions and perspectives -- MALDI-TOF MS for Environmental Analysis, Microbiome Research and as a Tool for Biological Resource Centres -- Introduction -- MALDI-TOF MS for microbiome analysis: microbial culturomics -- MALDI-TOF MS profiling in environmental analysis -- MALDI-TOF MS and Biological Resource Centres (BRC): the example of the Collection de l'Institut Pasteur (CIP) -- Strain identification within the Collection de l'Institut Pasteur (CIP) -- 8. The World of Nucleic Acid-based Mass Spectrometry for Microbial and Viral Detection -- Introduction -- Nucleic Acid MALDI-TOF MS -- PCR/ESI-MS -- Outlook -- 9. Future Trends and Perspectives of MALDI-TOF MS in the Microbiology Laboratory -- Introduction -- Improvement of current MALDI-TOF MS technologies -- Immuno-MALDI and imaging mass spectrometry: potential for further value of MALDI-TOF MS in microbiology -- Index.

In this timely and up-to-date book, experts in the field provide an overview of the application of MALDI-TOF MS in key areas of microbiology and discuss the impact this modern technology is having on laboratory practice and patient outcome. Several chapters cover applications in clinical and veterinary diagnostic laboratories, food microbiology, environmental microbiology and strain collections. Further chapters discuss the utilization of MALDI-TOF MS for the analysis of challenging microbial groups such as yeast and anaerobic bacteria. In addition, new applications such as microbial typing, DNA analysis and the detection of antibiotic resistance are also covered. The final chapter provides a valuable overview of potential future trends and developments in MALDI-TOF MS and assesses the impact of the technology in microbiology. This authoritative volume will be indispensable for all microbiology laboratories.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.