Methods in Biotechnology.

Intro -- Title Page -- Copyright -- Table of Contents -- Preface -- Acknowledgements -- Introduction to biotechnology lab -- Lab safety -- Good work habits -- Lab etiquette -- Lab record -- Lab report and assignment -- Further reading -- Laboratory Schedule (Methods in Biotechnology (MB)) -- Laboratory Schedule (Advanced Methods in Biotechnology (AMB) 1) -- Laboratory Schedule (AMB 2) -- About the companion website -- Part I: Methods in Biotechnology (MB) Laboratory Exercises -- Chapter 1: MB experiment 1: Lab measurements -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Pipetting skill -- Part II. Density and specific gravity -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 2: MB experiment 2: Use of the spectrophotometer and Beer's law -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 3: MB experiment 3: Making solutions and buffer efficacy -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. pH meter calibration -- Part II. Making solutions (to share four groups) -- Part III. Buffering capacity (addition of strong acid and strong base to buffered and unbuffered solutions) -- Part IV. Dilution effect on buffer pH and buffer capacity -- Discussion -- Post-lab assignment -- Further reading -- Chapter 4: MB experiment 4: Acid-base titration -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Two-point calibration of pH Meter -- Part II. Titration of acetic acid with NaOH -- Part III. Titration of HCl with NaOH -- Part IV. Data integration -- Discussion -- Post-lab assignment -- Further reading -- Chapter 5: MB experiment 5: Protein denaturation and precipitation -- Introduction -- Pre-lab assignment.

Materials and equipment -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 6: MB experiment 6: Bacterial transformation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Procedure (day 3) -- Discussion -- Post-lab assignment -- Further reading -- Chapter 7: MB experiment 7: GFP purification -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Discussion -- Further reading -- Chapter 8: MB experiment 8: SDS-PAGE analysis -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 9: MB experiment 9: DNA isolation -- Part I. Plasmid DNA isolation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Plasmid DNA isolation -- Part II. Genomic DNA isolation -- Introduction -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 10: MB experiment 10: PCR-based Alu-human DNA typing -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part E. Viewing and photography -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 11: MB experiment 11: Restriction enzyme digestion -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Chapter 12: MB experiment 12: Agarose gel electrophoresis -- Introduction -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 13: MB experiment 13: Ouchterlony and ELISA immunoassays -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Ouchterlony (double diffusion) assay -- Part II. ELISA assay -- Result -- Discussion -- Post-lab assignment -- Further reading.

Chapter 14: MB experiment 14: Testing plant substances for antimicrobial activity -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 15: MB experiment 15: Peroxidase enzyme activity assay -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Results -- Discussion -- Post-lab assignment -- References -- Part II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises -- chapter 16: AMB 1 experiment 16: Aseptic technique and culture handling -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- chapter 17: AMB 1 experiment 17: Yeast culture media preparation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- chapter 18: AMB 1 experiment 18: Growth curve -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 19: AMB 1 experiment 19: Mini plasmid prep -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (adapted from QIAprep miniprep kit) -- Post-lab assignment -- Additional information -- References -- Further reading -- Chapter 20: AMB 1 experiment 20: Restriction digestion, purification, concentration, and quantification of DNA -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 21: AMB 1 experiment 21: Polymerase chain reaction (PCR) -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Additional background information -- Further reading -- Chapter 22: AMB 1 experiment 22: TA, blunt end, SLIC, and CPEC cloning of PCR product -- Introduction.

Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Post-lab assignment -- Further reading -- Chapter 23: AMB 1 experiment 23: One-step multifragment assembly cloning -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Procedure (day 3) -- J. Screening of transformants -- Post-lab assignment -- References -- Further reading -- Chapter 24: AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Additional background information -- References -- Further reading -- chapter 25: AMB 1 experiment 25: Southern blot transfer -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Post-lab assignment -- Reference -- Further reading -- Chapter 26: AMB 1 experiment 26: Probe labeling and purification -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 27: AMB 1 experiment 27: Dot blot assay -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 28: AMB 1 experiment 28: Pre-hybridization, hybridization, and detection -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 29: AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (adapted from SV total isolation kit, Promega and verso one-step RT PCR Kit, Life technologies) -- Post-lab assignment -- References -- Further reading -- Chapter 30: AMB 1 experiment 30: Yeast-based in vivo recombination cloning -- Introduction.

Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Isolation of overlapping DNA fragments (day 1) -- Part II. Yeast transformation (day 2) -- Further reading -- Chapter 31: AMB 1 experiment 31: Plasmid DNA isolation from yeast -- Introduction -- Materials and equipment -- Procedure -- References -- Chapter 32: AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA -- Introduction -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 33: AMB 1 experiment 33: X-gal filter lift assay -- Introduction -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 34: AMB 1 experiment 34: Protein quantitation assay -- Introduction -- Part I. Bradford assay -- Materials and equipment -- Procedure -- Part II. Lowry assay -- Materials and equipment -- Procedure -- Part III. BCA assay -- Material and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 35: AMB 1 experiment 35: Quantitative β-Galactosidase assay in yeast -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Permeabilized cell assay -- Part II. Cell-free protein extract assay -- Post-lab assignment -- Reference -- Further reading -- Chapter 36: AMB 1 experiment 36: Gel filtration chromatography (GFC) -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 37: AMB 1 experiment 37: Ion exchange chromatography (IEC) -- Background -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Part III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises -- Chapter 38: AMB 2 experiment 38: E. coli culture media preparation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure.

Post-lab assignment.

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