Drosophila Protocols.

Intro -- Contents -- Preface -- Abbreviations -- Preparation and Analysis of Drosophila Mitotic Chromosomes -- In Situ Hybridization to Somatic Chromosomes -- BrdU Labeling of Chromosomes -- Analysis of Meiosis in Fixed and Live Oocytes by Light Microscopy -- Cytological Analysis of Spermatocyte Growth and Male Meiosis in Drosophila melanogaster -- Preparation and Analysis of Polytene Chromosomes -- In Situ Hybridization to Polytene Chromosomes -- Mapping Protein Distributions on Polytene Chromosomes by Immunostaining -- Fluorescent Analysis of Drosophila Embryos -- Imaginal Discs -- The Neuromuscular Junction -- Histological Techniques for the Drosophila Eye Part I: Larva and Pupa -- Histological Techniques for the Drosophila Eye Part II: Adult -- Preparation of Thin Sections of Drosophila for Examination by Transmission Electron Microscopy -- Electrophysiological Approaches to the Neuromusculature -- Functional Assays of the Peripheral and Central Nervous Systems -- GFP and Other Reporters -- Quantitative Microinjection of Drosophila Embryos -- Targeted Disruption of Gene Function in Drosophila by RNA Interference -- Drosophila Cell Culture and Transformation -- Generating Antibodies against Drosophila Proteins -- Photoactivated Gene Expression for Cell- fate Mapping and Cell Manipulation -- Recovery of DNA Sequences Flanking P- element Insertions: Inverse PCR and Plasmid Rescue -- GAL4- mediated Ectopic Gene Expression in Drosophila -- Functional Cell Ablation -- Preparation of DNA from E. coli Cells Containing Bacteriophage P1 Clones -- Drosophila Microarrays: From Arrayer Construction to Hybridization -- Using Drosophila Genome Databases -- Preparation and Analysis of High- molecular- weight DNA in Drosophila -- Culturing Large Populations of Drosophila for Protein Biochemistry*.

Preparation of Nuclear Extracts from Drosophila Embryos and In Vitro Transcription Analysis -- Preparation of Membrane Proteins from Drosophila Embryos -- Preparing Cytoplasmic Extracts from Drosophila Embryos -- Immunoblotting of Proteins from Single Drosophila Embryos -- Laboratory Culture of Drosophila -- Preparation of Larval and Adult Cuticles for Light Microscopy -- Exposing Drosophila to Neuroactive Drugs -- 1989 Table of Contents from Drosophila: A Laboratory Manual -- Anatomical Drawings of Drosophila -- Solutions and Reagents -- Cautions -- Suppliers -- Trademarks -- Index.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.