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Pathogen Detection Methods : Biosensor Development.

By: Contributor(s): Material type: TextTextSeries: Biotechnology in Agriculture, Industry and MedicinePublisher: Hauppauge : Nova Science Publishers, Incorporated, 2010Copyright date: ©2010Edition: 1st edDescription: 1 online resource (156 pages)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781616686994
Subject(s): Genre/Form: Additional physical formats: Print version:: Pathogen Detection Methods: Biosensor DevelopmentDDC classification:
  • 610.28/4
LOC classification:
  • R857.B54 -- B35 2010eb
Online resources:
Contents:
Intro -- PATHOGEN DETECTION METHODS: BIOSENSOR DEVELOPMENT -- PATHOGEN DETECTION METHODS: BIOSENSOR DEVELOPMENT -- CONTENTS -- PREFACE -- LIST OF ABBREVIATIONS -- Chapter 1 PATHOGENS AT THE HUMAN ENVIRONMENT -- ABSTRACT -- 1.1. WATERBORNE PATHOGENS -- 1.2. FOODBORNE PATHOGENS -- REFERENCES -- Chapter 2 CONVENTIONAL AND RAPID METHODS FOR PATHOGEN DETECTION -- ABSTRACT -- 2.1. CULTURE-BASED METHODS -- 2.2. ENZYMATIC ASSAYS AND IMMUNOASSAYS -- 2.3. NUCLEIC ACID-BASED DIAGNOSTICS -- REFERENCES -- Chapter 3 BIOSENSORS: AN ALTERNATIVE TO TRADITIONAL METHODS -- ABSTRACT -- 3.1. BIORECOGNITION ELEMENTS IN SENSOR BIOENGINEERING: THE CLUE FOR BIOSENSOR SPECIFICITY -- 3.1.1. Protein Based Biorecognition Elements -- 3.1.1.1. Antibodies -- 3.1.1.2. Bacteriophages -- 3.1.1.3. Lectins -- 3.1.1.4. Antimicrobial Peptides -- 3.1.2. Nucleic Acid Biorecognition Elements -- 3.1.2.1. Nucleic Acid Probes -- 3.1.2.2. Peptide Nucleic Acids -- 3.1.2.3. Aptamers -- 3.1.3. Whole Bacterial Cells as Biorecognition Elements -- 3.1.4. Biomimetic Receptors -- 3.2. FUNCTIONALISATION OF THE SENSING SURFACE: TOWARDS BIOSENSOR SPECIFICITY AND ROBUSTNESS -- 3.2.1. Bioreceptor Random Physisorption -- 3.2.2. Self Assembled Monolayers (SAM) and Bioreceptor Self Assembly onto Metal Surfaces -- 3.2.3. Silanisation of Glass and Silica Derivatives -- 3.2.4. Chemical Conjugation -- 3.2.5. Functionalisation by Biocomponent Entrapment -- 3.2.6. Bioreceptor Capture by Affinity Proteins -- 3.2.6.1. Protein A and Protein G -- 3.2.6.2. The Biotin-(Strept)Avidin System -- 3.2.7. Surface Physical Blocking and the Inclusion of Negative Controls -- 3.3. TRANSDUCTION FORMATS USED FOR PATHOGEN DETECTION: SENSITIVITY OF THE BIOSENSOR -- 3.3.1. Electrochemical Transduction -- 3.3.1.1. Amperometric Biosensors -- 3.3.1.2. Impedimetric Biosensors -- 3.3.1.3. Conductimetric Biosensors.
3.3.1.4. Potentiometric Biosensors -- 3.3.2. Optical Transduction -- 3.3.2.1. Fiber-Optic Biosensors -- 3.3.2.2. Microarray Biosensors -- 3.3.2.3. Surface Plasmon Resonance (SPR) -- 3.3.2.4. Evanescence Waveguide Biosensors -- 3.3.3. Calorimetric Transduction -- 3.3.4. Mass-Sensitive and Resonant Sensors -- 3.3.4.1. Quartz Crystal Resonators and Quartz Crystal Microbalance -- 3.3.4.2. Surface Acoustic Wave (SAW) Sensors -- 3.3.4.3. Cantilevers -- REFERENCES -- Chapter 4 BIOSENSORS FOR PATHOGEN DETECTION -- 4.1. DETECTION OF BACTERIAL METABOLISM AND/OR BACTERIAL ENZYMES -- 4.2. DETECTION OF WHOLE CELLS OR CELL LYSATES -- 4.2.1. Sandwich-Based Biosensors -- 4.2.2. One-Step Reagentless Biosensors -- 4.3. BIOSENSORS FOR THE DETECTION OF BACTERIAL NUCLEIC ACIDS -- 4.3.1. Optical Detection -- 4.3.2. Electrochemical Detection -- 4.3.3. Piezoelectric Genosensors -- 4.4. DETECTION OF TOXINS -- 4.5. MULTIPLEX DETECTION -- REFERENCES -- Chapter 5 DRAWBACKS AND FUTURE TRENDS OF PATHOGEN BIOSENSORS -- REFERENCES -- INDEX.
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Intro -- PATHOGEN DETECTION METHODS: BIOSENSOR DEVELOPMENT -- PATHOGEN DETECTION METHODS: BIOSENSOR DEVELOPMENT -- CONTENTS -- PREFACE -- LIST OF ABBREVIATIONS -- Chapter 1 PATHOGENS AT THE HUMAN ENVIRONMENT -- ABSTRACT -- 1.1. WATERBORNE PATHOGENS -- 1.2. FOODBORNE PATHOGENS -- REFERENCES -- Chapter 2 CONVENTIONAL AND RAPID METHODS FOR PATHOGEN DETECTION -- ABSTRACT -- 2.1. CULTURE-BASED METHODS -- 2.2. ENZYMATIC ASSAYS AND IMMUNOASSAYS -- 2.3. NUCLEIC ACID-BASED DIAGNOSTICS -- REFERENCES -- Chapter 3 BIOSENSORS: AN ALTERNATIVE TO TRADITIONAL METHODS -- ABSTRACT -- 3.1. BIORECOGNITION ELEMENTS IN SENSOR BIOENGINEERING: THE CLUE FOR BIOSENSOR SPECIFICITY -- 3.1.1. Protein Based Biorecognition Elements -- 3.1.1.1. Antibodies -- 3.1.1.2. Bacteriophages -- 3.1.1.3. Lectins -- 3.1.1.4. Antimicrobial Peptides -- 3.1.2. Nucleic Acid Biorecognition Elements -- 3.1.2.1. Nucleic Acid Probes -- 3.1.2.2. Peptide Nucleic Acids -- 3.1.2.3. Aptamers -- 3.1.3. Whole Bacterial Cells as Biorecognition Elements -- 3.1.4. Biomimetic Receptors -- 3.2. FUNCTIONALISATION OF THE SENSING SURFACE: TOWARDS BIOSENSOR SPECIFICITY AND ROBUSTNESS -- 3.2.1. Bioreceptor Random Physisorption -- 3.2.2. Self Assembled Monolayers (SAM) and Bioreceptor Self Assembly onto Metal Surfaces -- 3.2.3. Silanisation of Glass and Silica Derivatives -- 3.2.4. Chemical Conjugation -- 3.2.5. Functionalisation by Biocomponent Entrapment -- 3.2.6. Bioreceptor Capture by Affinity Proteins -- 3.2.6.1. Protein A and Protein G -- 3.2.6.2. The Biotin-(Strept)Avidin System -- 3.2.7. Surface Physical Blocking and the Inclusion of Negative Controls -- 3.3. TRANSDUCTION FORMATS USED FOR PATHOGEN DETECTION: SENSITIVITY OF THE BIOSENSOR -- 3.3.1. Electrochemical Transduction -- 3.3.1.1. Amperometric Biosensors -- 3.3.1.2. Impedimetric Biosensors -- 3.3.1.3. Conductimetric Biosensors.

3.3.1.4. Potentiometric Biosensors -- 3.3.2. Optical Transduction -- 3.3.2.1. Fiber-Optic Biosensors -- 3.3.2.2. Microarray Biosensors -- 3.3.2.3. Surface Plasmon Resonance (SPR) -- 3.3.2.4. Evanescence Waveguide Biosensors -- 3.3.3. Calorimetric Transduction -- 3.3.4. Mass-Sensitive and Resonant Sensors -- 3.3.4.1. Quartz Crystal Resonators and Quartz Crystal Microbalance -- 3.3.4.2. Surface Acoustic Wave (SAW) Sensors -- 3.3.4.3. Cantilevers -- REFERENCES -- Chapter 4 BIOSENSORS FOR PATHOGEN DETECTION -- 4.1. DETECTION OF BACTERIAL METABOLISM AND/OR BACTERIAL ENZYMES -- 4.2. DETECTION OF WHOLE CELLS OR CELL LYSATES -- 4.2.1. Sandwich-Based Biosensors -- 4.2.2. One-Step Reagentless Biosensors -- 4.3. BIOSENSORS FOR THE DETECTION OF BACTERIAL NUCLEIC ACIDS -- 4.3.1. Optical Detection -- 4.3.2. Electrochemical Detection -- 4.3.3. Piezoelectric Genosensors -- 4.4. DETECTION OF TOXINS -- 4.5. MULTIPLEX DETECTION -- REFERENCES -- Chapter 5 DRAWBACKS AND FUTURE TRENDS OF PATHOGEN BIOSENSORS -- REFERENCES -- INDEX.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.

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