Pathogenic Escherichia coli : Evolution, Omics, Detection and Control.

Intro -- Contents -- Preface -- 1. Escherichia coli Pathotypes -- Introduction -- Enteroinvasive Escherichia coli (EIEC) -- Diffusely adherent E. coli (DAEC) -- Enteropathogenic E. coli (EPEC) -- Enteroaggregative E. coli (EAEC) -- Enterotoxigenic E. coli (ETEC) -- Shiga toxin-producing E. coli (STEC) -- Enteroaggregative haemorrhagic E. coli (EAHEC) -- Adherent invasive E. coli (AIEC) -- Extraintestinal pathogenic E. coli (ExPEC) -- Hybrid pathogenic E. coli -- Evolution of pathogenic E. coli -- Control of pathogenic E. coli -- 2. Escherichia coli Genome Plasticity and Evolution -- Introduction -- The E. coli phylogroups -- Development of a genomic framework for the E. coli species -- Hallmark features of the species landscape -- Intraphylogroup genomic diversity -- Refinement of O157:H7 cladistics -- Genome architecture and plasticity framework -- Genome architecture and plasticity in phylogroup E -- Genome architecture and plasticity in phylogroup B1 -- Gap analysis and landscape bias: future directions -- 3. Diarrhoeagenic Escherichia coli: Virulence Genes and Other Markers for Detection and Typing -- Diagnostic markers for enterotoxigenic E. coli (ETEC) -- Diagnostic markers for enteroinvasive E. coli (EIEC) -- Diagnostic markers for enteraggregative E. coli (EAEC) -- Diagnostic markers for enteropathogenic E. coli (EPEC) -- Diagnostic and typing markers for Shiga toxin-producing E. coli (STEC) -- 4. Extra-intestinal Pathogenic Escherichia coli (ExPEC): Characteristics, Virulence Genes, Detection and Control -- Introduction -- ExPEC-associated clinical diseases -- Challenges in predicting virulence of individual ExPEC strains -- Multidrug resistance amongst ExPEC -- Successful clones -- Control of ExPEC infections -- Detection and characterization -- Future trends and challenges.

5. Bacteriophage-based Strategies to Control Pathogenic Escherichia coli in Humans and Animals -- Introduction -- Therapeutic use of bacteriophages -- Pre-harvest strategies to reduce pathogenic E. coli -- Human trials -- Future trends -- Conclusion -- 6. New Developments in Detection Technologies for Escherichia coli and Other Pathogenic Organisms -- Colorimetric detection methods -- Spectroscopic detection methods -- Paper-based detection methods -- Chip-based detection methods -- 7. Understanding Pathogenic Escherichia coli Through Whole-genome Sequencing -- Introduction -- Genomics to understand the continuum of pathogenic E. coli -- Hybrid shigatoxigenic E. coli -- WGS-based typing of STEC for surveillance and outbreak investigation -- 8. Use of Whole-genome Sequencing to Improve Investigations of Outbreaks of Escherichia coli -- Historical perspective -- Current methods for typing E. coli using WGS data -- Outbreak definitions and outbreak detection using SNP typing -- Initial evaluation studies -- Comparison of WGS with current molecular typing techniques -- Robust and timely phylogenetic resolution improves case ascertainment during outbreak -- Outbreaks associated with direct contact with animals -- Inferring the geographical origin of an isolate from the WGS phylogeny at the national level -- Food-borne outbreaks - domestic or non-domestic produce? -- Outbreaks caused by STEC other than serotype O157:H7 -- Outbreaks of DEC other than STEC -- Use of long-read sequencing in outbreak investigations -- Summary -- 9. Data Processing of Escherichia coli Genome Sequencing, Characterization and Comparison -- Introduction -- Escherichia coli: an introduction -- Advances in DNA sequencing technologies -- File formats -- Genome assembly and annotation -- In silico characterization of Escherichia coli -- Comparison and visualization of genetic content.

Pan-genome characterization and phylogenetic tools -- Future perspectives -- 10. Culture-independent Sequence-based Approaches for Diagnostics and Food Safety Testing -- Introduction -- Approaches to sequencing and analysis of metagenomic samples -- Microbial community analysis applications -- Strain characterization using shotgun metagenomics -- Determining strain relatedness using metagenomic data -- Detection sensitivity -- Challenges and future directions -- 11. Use of Omics Technologies to Develop Strategies to Control Escherichia coli from Farm to Table -- Shiga toxin-producing E. coli (STEC) as a public health concern -- STEC stress responses characterized by omics technologies -- STEC response to stresses in the primary production environment -- STEC responses to stresses in foods -- STEC responses to stresses encountered during food processing -- Using microbiome data to characterize interactions between STEC and other bacteria -- Using omics data for intervention strategies -- Future trends -- 12. Application of Omics Technologies for in Silico Modelling to Understand Stress-triggered Physiology of Escherichia coli and to Develop Novel Therapeutics -- Introduction -- Background Information -- Methods used -- Results -- Conclusions and discussion -- Current Books of Interest -- Index.

Under the expert guidance of the editors in this book, renowned international authors provide timely and up-to-date reviews of current cutting-edge E. coli omics, molecular- and cellular-biology research. Topics range from E. coli genome plasticity and evolution to the application of omics technologies for in silico modeling to understand stress-triggered physiological responses. This authoritative volume is essential reading for scientists, both experts and students, working on pathogenic E. coli in academia, government, and biotechnology companies. It is also a must-read for anyone with an interest in bacterial pathogenesis and an important acquisition for all microbiology libraries.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.