Molecular Methods in Plant Disease Diagnostics : Principles and Protocols.

Cover -- Half Title -- Title -- Copyright -- Contents -- Editors and Contributors -- Foreword -- Chapter 1 Introduction: advances in plant diagnostics - historical perspectives and future directions -- 1.1 Progress in recent years -- 1.2 The rise and rise of PCR -- 1.3 Quality matters2 -- 1.4 The move from lab to field -- 1.5 Identifying the unknown -- 1.6 Future challenges -- Chapter 2 Conventional PCR -- 2.1 Introduction -- 2.2 Viruses and viroids -- 2.3 Phytoplasmas9 -- 2.4 Bacterial pathogens -- 2.5 PCR detection of fungi -- 2.6 Internal controls -- 2.7 PCR optimization -- Chapter 3 Real-time PCR -- 3.1 Introduction -- 3.2 Coupling PCR amplification with the generation of fluorescence -- 3.3 Probe-based real-time PCR -- 3.4 Non-probe chemistries -- 3.5 Reaction components and cycling programmes -- 3.6 Real-time PCR instruments -- 3.7 Selecting and designing an assay -- 3.8 Sample preparation and controls -- 3.9 Assay performance and validation -- 3.10 Qualitative and quantitative real-time PCR -- 3.11 Multiplex real-time PCR -- 3.12 Confirming the real-time PCR result -- Chapter 4 Loop-mediated isothermal amplification (LAMP) -- 4.1 Introduction -- 4.2 Loop-mediated isothermal amplification (LAMP) -- 4.3 LAMP detection methods -- 4.4 LAMP primer design -- 4.5 Other isothermal methods -- Chapter 5 DNA barcoding for identification of plant pathogens -- 5.1 Introduction -- 5.2 The application of DNA barcoding -- 5.3 Technical considerations -- 5.4 Analytical considerations -- 5.5 Barcoding plant pathogens -- Chapter 6 DNA barcoding of invertebrate plant pests -- 6.1 Introduction -- 6.2 Barcoding nematodes -- 6.3 Barcoding arthropods -- 6.4 Conclusions and outlook -- Chapter 7 Microarrays125 -- 7.1 Introduction -- 7.2 Microarrays for pathogen detection -- 7.3 Alternative microarray platforms -- 7.4 Array design -- 7.5 Conclusions.

Chapter 8 Next-generation sequencing -- 8.1 Introduction -- 8.2 Total RNA sequence generation -- 8.3 Quality control -- 8.4 Metatranscriptomics for pathogen detection and viral whole genome sequencing -- Chapter 9 On-site testing for plant pathogens -- 9.1 Introduction -- 9.2 On-site testing using antibody-based methods -- 9.3 On-site nucleic acid extraction -- 9.4 Real-time PCR in the field -- 9.5 Isothermal amplification in the field -- 9.6 Deployment of tests in the field -- Chapter 10 Quality assurance for molecular testing in plant health -- 10.1 Introduction -- 10.2 Fit for purpose testing -- 10.3 Selection of molecular tests -- 10.4 Validation of molecular tests -- 10.5 Implementation of a molecular test in terms of quality -- 10.6 Infrastructure and equipment -- 10.7 Critical reagents -- 10.8 Measurement uncertainty -- 10.9 Documentation183 -- 10.10 Survey of test performance and quality management -- 10.11 Continuous improvement -- 10.12 Conclusion -- Index -- Back.

Molecular diagnostic of plant diseases have helped by increasing the efficacy, accuracy or speed of diagnosis, while their common technological basis also reduces reliance on staff with very specialist skills. This book provides protocols for the majority of nucleic acid-based methods applied to plant pathogen detection and identification.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.