Corynebacterium Glutamicum : From Systems Biology to Biotechnological Applications.

Intro -- Contents -- Contributors -- Current Books of Interest -- Preface -- 1: Trends in Corynebacterium glutamicum Research and Application -- From glutamate producer to a biotechnology workhorse -- Systems biology -- Corynebacterium glutamicum as a synthetic biology platform -- Corynebacterium glutamicum-based green technology -- 2: Proteomics of Corynebacterium glutamicum -- Introduction -- Understanding Corynebacterium glutamicum physiology with proteomics: application examples -- Methods of Corynebacterium glutamicum proteomics -- Conclusion and outlook -- 3: Developing Interpretation of Intracellular Metabolism of Corynebacterium glutamicum by Using Flux Analysis Technology -- Introduction -- Understanding the physiology of Corynebacterium glutamicum lysine production and glutamate production through conventional metabolic flux analysis -- Fractional 13C enrichment-based metabolic flux analysis -- Improvement in metabolic flux analysis precision -- Conclusion -- 4: Growth and Production Capabilities of Corynebacterium glutamicum: Interrogating a Genome-scale Metabolic Network Model -- Introduction -- The metabolic network of Corynebacterium glutamicum -- Stoichiometric modelling fundamentals -- Model validation -- Predicting production capabilities for amino acids -- Uncertainties in metabolic network models -- Metabolic flux analysis -- Conclusions -- 5: Metabolic Engineering of Corynebacterium glutamicum for Alternative Carbon Source Utilization -- Introduction -- Engineering of Corynebacterium glutamicum for alternative carbon sources -- Complex carbon sources -- Summary and outlook -- 6: Manipulation of Nitrogen Metabolism and Alternative Nitrogen Sources for Corynebacterium glutamicum -- Ammonium assimilation in Corynebacterium glutamicum -- Regulation of nitrogen metabolism.

Manipulation of nitrogen metabolism for amino acid production -- Overexpression, deletion and heterologous expression of glutamate dehydrogenase -- Overexpression of glutamine synthetases -- Influence of glutamate synthase on L-glutamate biosynthesis -- Changing ammonium assimilation and amino acid production by manipulation of α-ketoglutarate supply -- Influence of ammonium and glutamate transport systems on amino acid production -- Manipulation of nitrogen regulation: influences on metabolite pools -- Assimilation of alternative nitrogen sources -- Concluding remarks -- 7: Transport, Degradation and Assimilation of Aromatic Compounds and their Regulation in Corynebacterium glutamicum -- Introduction -- What do the Corynebacterium glutamicum genomes predict for degradation and assimilation of aromatic compounds? -- Corynebacterium glutamicum grows on various aromatic compounds -- Physiological adaptation of Corynebacterium glutamicum growing on aromatic compounds compared with carbohydrates -- Uptake and transport of aromatic compounds in Corynebacterium glutamicum -- Aromatic compounds degraded via protocatechuate branch of the β-ketoadipate pathway -- Aromatic compounds degraded via the catechol branch of the β-ketoadipate pathway -- Degradation of 3-hydroxybenzoate and gentisate: the mycothiol-dependent gentisate pathway -- Degradation of resorcinol and 2,4-dihydroxybenzoate via hydroxyquinol 1,2-dioxygenase cleavage -- Metabolism of phenylacetate via phenylacetyl-CoA pathway -- Regulation of aromatic metabolism in Corynebacterium glutamicum -- Perspectives -- 8: Engineering Corynebacterium glutamicum for the Production of Organic Acids and Alcohols -- Introduction -- Metabolic engineering of Corynebacterium glutamicum for organic acid production -- Metabolic engineering of Corynebacterium glutamicum for alcohol production -- Concluding remarks.

9: Microbial Factory for the Production of Polyesters: A New Platform of Corynebacterium glutamicum -- Introduction -- Polyhydroxyalkanoate production in Corynebacterium glutamicum -- Poly(lactic acid)-like polyester production in Corynebacterium glutamicum -- Poly(3-hydroxybutyrate) production from starch based on Corynebacterium glutamicum with cell surface displaying of α-amylase -- Conclusion -- 10: Biotechnological Application of Corynebacterium glutamicum Under Oxygen Deprivation -- Introduction -- Basics of the bioprocess: utilization of Corynebacterium glutamicum under oxygen deprivation -- Metabolic engineering Corynebacterium glutamicum for production of value-added chemicals via the growth-arrested bioprocess -- Application of the growth-arrested bioprocess to lignocellulosic biorefinery -- Future trends -- 11: Corynebacterium glutamicum as a Platform Organism for the Secretory Production of Heterologous Proteins -- Introduction -- Protein export systems of Corynebacterium glutamicum -- Secretory production of heterologous proteins by Corynebacterium glutamicum -- Concluding remarks -- 12: Genetically Encoded Biosensors for Strain Development and Single-cell Analysis of Corynebacterium glutamicum -- Introduction: biosensors -- Design of biosensors based on regulatory circuits -- Characterization of biosensor performance parameters -- Biosensors in Corynebacterium glutamicum -- Application of biosensors in screening and single-cell analysis -- Future perspectives and concluding remarks -- Index.

This book provides a comprehensive overview of current knowledge and research on C. glutamicum systems biology and biotechnological applications. Written by a team of prominent scientists under the expert editorship of Andreas Burkovski, the topics covered include: proteomics; flux analysis technology for metabolic analysis; metabolic engineering for alternative carbon source utilization; manipulation of nitrogen metabolism; transport, degradation and assimilation of aromatic compounds and their regulation; engineering for production of organic acids and alcohols; microbial factory for the production of polyesters; biotechnological application under oxygen deprivation; the secretory production of heterologous proteins; genetically-encoded biosensors. Packed with practical information and state-of-the-art science this concise volume is an essential handbook for everyone working with Corynebacterium and related organisms in academia, biotechnology companies, and the pharmaceutical industry and is a recommended volume for all microbiology libraries.

Description based on publisher supplied metadata and other sources.

Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.