Gene Cloning and DNA Analysis : An Introduction.

Intro -- Title page -- Copyright -- Preface to the Seventh Edition -- About the companion website -- Part I The Basic Principles of Gene Cloning and DNA Analysis -- Chapter 1 Why Gene Cloning and DNA Analysis are Important -- 1.1 The early development of genetics -- 1.2 The advent of gene cloning and the polymerase chain reaction -- 1.3 What is gene cloning? -- 1.4 What is PCR? -- 1.5 Why gene cloning and PCR are so important -- 1.6 How to find your way through this book -- Further reading -- Chapter 2 Vectors for Gene Cloning: Plasmids and Bacteriophages -- 2.1 Plasmids -- 2.2 Bacteriophages -- Further reading -- Chapter 3 Purification of DNA from Living Cells -- 3.1 Preparation of total cell DNA -- 3.2 Preparation of plasmid DNA -- 3.3 Preparation of bacteriophage DNA -- Further reading -- Chapter 4 Manipulation of Purified DNA -- 4.1 The range of DNA manipulative enzymes -- 4.2 Enzymes for cutting DNA: Restriction endonucleases -- 4.3 Ligation: Joining DNA molecules together -- Further reading -- Chapter 5 Introduction of DNA into Living Cells -- 5.1 Transformation: The uptake of DNA by bacterial cells -- 5.2 Identification of recombinants -- 5.3 Introduction of phage DNA into bacterial cells -- 5.4 Identification of recombinant phages -- 5.5 Introduction of DNA into non-bacterial cells -- Further reading -- Chapter 6 Cloning Vectors for Escherichia coli -- 6.1 Cloning vectors based on E. coli plasmids -- 6.2 Cloning vectors based on λ bacteriophage -- 6.3 Cloning vectors for the synthesis of single-stranded DNA -- 6.4 Vectors for other bacteria -- Further reading -- Chapter 7 Cloning Vectors for Eukaryotes -- 7.1 Vectors for yeast and other fungi -- 7.2 Cloning vectors for higher plants -- 7.3 Cloning vectors for animals -- Further reading -- Chapter 8 How to Obtain a Clone of a Specific Gene -- 8.1 The problem of selection.

8.2 Direct selection -- 8.3 Identification of a clone from a gene library -- 8.4 Methods for clone identification -- Further reading -- Chapter 9 The Polymerase Chain Reaction -- 9.1 PCR in outline -- 9.2 PCR in more detail -- 9.3 After the PCR: Studying PCR products -- 9.4 Real-time PCR enables the amount of starting material to be quantified -- Further reading -- Part II The Applications of Gene Cloning and DNA Analysis in Research -- Chapter 10 Sequencing Genes and Genomes -- 10.1 Chain-termination DNA sequencing -- 10.2 Next-generation sequencing -- 10.3 How to sequence a genome -- Further reading -- Chapter 11 Studying Gene Expression and Function -- 11.1 Studying the RNA transcript of a gene -- 11.2 Studying the regulation of gene expression -- 11.3 Identifying and studying the translation product of a cloned gene -- Further reading -- Chapter 12 Studying Genomes -- 12.1 Genome annotation -- 12.2 Studies of the transcriptome and proteome -- Further reading -- Part III The Applications of Gene Cloning and DNA Analysis in Biotechnology -- Chapter 13 Production of Protein from Cloned Genes -- 13.1 Special vectors for the expression of foreign genes in E. coli -- 13.2 General problems with the production of recombinant protein in E. coli -- 13.3 Production of recombinant protein by eukaryotic cells -- Further reading -- Chapter 14 Gene Cloning and DNA Analysis in Medicine -- 14.1 Production of recombinant pharmaceuticals -- 14.2 Identification of genes responsible for human diseases -- 14.3 Gene therapy -- Further reading -- Chapter 15 Gene Cloning and DNA Analysis in Agriculture -- 15.1 The gene addition approach to plant genetic engineering -- 15.2 Gene subtraction -- 15.3 Problems with genetically modified plants -- Further reading -- Chapter 16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology.

16.1 DNA analysis in the identification of crime suspects -- 16.2 Studying kinship by DNA profiling -- 16.3 Sex identification by DNA analysis -- 16.4 Archaeogenetics: Using DNA to study human prehistory -- Further reading -- Glossary -- Index -- EULA.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.