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Polymerase Chain Reaction : Theory and Technology.

By: Contributor(s): Material type: TextTextPublisher: Norfolk : Caister Academic Press, 2019Copyright date: ©2019Edition: 1st edDescription: 1 online resource (269 pages)Content type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
ISBN:
  • 9781912530250
Subject(s): Genre/Form: Additional physical formats: Print version:: Polymerase Chain ReactionDDC classification:
  • 547.28
LOC classification:
  • QP606.D46 .B445 2019
Online resources:
Contents:
Intro -- Contents -- Chapter 1 Introduction to the Real-time Polymerase Chain Reaction -- Chapter 2 Principles of the Real-time Polymerase Chain Reaction -- Chapter 3 Homogenous Fluorescent Chemistries for Real-time PCR -- Chapter 4 Instrumentation and Fluorescent Chemistries Used in Quantitative Polymerase -- Chapter 5 Quantification Strategies in Real-time Polymerase Chain Reaction -- Chapter 6 The Extraction and Purification of Nucleic Acids for Analysis by PCR -- Chapter 7 Sample Preparation for Real-time PCR in Food Science -- Chapter 8 Internal and Other Controls for Real-time PCR Validation -- Chapter 9 Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase -- Chapter 10 Internal Amplification Controls in Real-time Polymerase Chain Reaction-Based -- Chapter 11 Standardization of Real-time PCR Methods in Food Microbiology -- Chapter 12 MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay -- Chapter 13 Analysis of mRNA Expression by Real-time PCR -- Chapter 14 Real-time PCR Arrays.
Summary: This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.
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Intro -- Contents -- Chapter 1 Introduction to the Real-time Polymerase Chain Reaction -- Chapter 2 Principles of the Real-time Polymerase Chain Reaction -- Chapter 3 Homogenous Fluorescent Chemistries for Real-time PCR -- Chapter 4 Instrumentation and Fluorescent Chemistries Used in Quantitative Polymerase -- Chapter 5 Quantification Strategies in Real-time Polymerase Chain Reaction -- Chapter 6 The Extraction and Purification of Nucleic Acids for Analysis by PCR -- Chapter 7 Sample Preparation for Real-time PCR in Food Science -- Chapter 8 Internal and Other Controls for Real-time PCR Validation -- Chapter 9 Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase -- Chapter 10 Internal Amplification Controls in Real-time Polymerase Chain Reaction-Based -- Chapter 11 Standardization of Real-time PCR Methods in Food Microbiology -- Chapter 12 MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay -- Chapter 13 Analysis of mRNA Expression by Real-time PCR -- Chapter 14 Real-time PCR Arrays.

This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.

Description based on publisher supplied metadata and other sources.

Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.

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