Transgenic Plants : Recent Developments.
- 1st ed.
- 1 online resource (157 pages)
- Botanical Research and Practices .
- Botanical Research and Practices .
Intro -- TRANSGENIC PLANTS -- TRANSGENIC PLANTS -- CONTENTS -- PREFACE -- PLANT LEAF DECOMPOSITION, DNA RELEASE, PERSISTENCE AND TRANSFER INTO THE ENVIRONMENT -- ABSTRACT -- 1. INTRODUCTION -- 2. FATE OF DNA INTO THE ENVIRONMENT -- 2.1. Fate of Extracellular DNA in Aquatic Environment -- 2.2. Persistence of Transgenic DNA in Aquatic Environment -- 3. PLANT MATERIAL DECOMPOSITION AND DNA RELEASE -- 4. WATER FLOW AND DNA TRANSPORT IN SUBSURFACE ENVIRONMENT -- 5. ANTIBIOTIC RESISTANCE BACTERIA AND HORIZONTAL GENE TRANSFER -- 6. A BRIEF PROTOCOL TO STUDY THE FATE OF RDNA IN TO THE AQUATIC ENVIRONMENT -- 6.1. Decrease of Transgenic DNA in Planta during Plant Material Decomposing in Sediments -- Plant Leaf Mass Loss -- Identity of Transgene in Planta -- 6.2. Persistence of Transgenic DNA in Water Column and Sediments -- 6.3. Antibiotic-resistant Bacteria and Horizontal Gene Transfer -- CONCLUSION -- REFERENCES -- ESTABLISHMENT OF LIGHT FORMULA AND LIGHT ENVIRONMENTAL MANAGEMENT STRATEGY FOR HIGH-EFFICIENT PLANT CULTIVATION WITH ARTIFICIAL LIGHT SOURCES -- ABSTRACT -- INTRODUCTION -- LIGHT FORMULA (LF): CONCEPT AND SIGNIFICANCE -- LIGHT-EMITTING DIODES (LEDS): AN OPTIMAL TOOL FOR LF RESEARCH -- LIGHT FORMULA (LF) FOR PROTECTED HORTICULTURAL PLANTS: PRIMARY CONCLUSION -- LF: AN IMPORTANT PART OF THE LIGHT ENVIRONMENT MANAGEMENT STRATEGY (LEMS) -- CONCLUSION -- ACKNOWLEDGMENTS -- REFERENCES -- HARVEST-INDUCIBLE GENES AND PROMOTERS IN ALFALFA -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- Plant Materials -- RNA Extraction -- Subtractive Hybridization -- Cloning of Harvest-Inducible cDNA Fragments -- Northern Blot Analysis -- Isolation Promoter of the hi12 Gene by Genomic Walking -- RESULTS -- Isolation of Harvest-Inducible cDNA Clones -- Northern Blot Analysis -- GUS Gene Construct with the hi12 Gene Promoter and Plant Transformation. GUS Gene Activity Driven by hi12 Promoter -- DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- TOXIC IMPACTS OF THREE VETERINARY ANTIBIOTICS ON SEED GERMINATION AND GROWTH AS WELL AS NUTRITIONAL QUALITY OF VEGETABLES -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- Materials -- Experiment 1 -- Experiment 2 -- RESULTS -- Impacts of Antibiotics on Root Elongation of Three Vegetables -- Impacts of TC on Root Elongation of Three Vegetables -- Impacts of OTC on Root Elongation of Three Vegetables -- Impacts of CTC on Root Elongation of Three Vegetables -- Impacts of Antibiotics on Growth and Quality Indices of Hydroponic Lettuce -- Impacts of CTC and OTC on Hydroponic Growth and Leafy SPAD of Lettuce -- Impacts of CTC and OTC on Soluble Sugar Content of Lettuce -- Impacts of CTC and OTC on Soluble Protein Content of Lettuce -- DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- GROWTH RESPONSES OF BT AND NON-BT COTTONS TO SOIL PHOSPHORUS, COPPER AND CADMIUM LEVELS -- ABSTRACT -- INTRODUCTION -- MATERIALS AND METHODS -- Plants and Soils -- Experimental Design -- Determination Methods -- Statistics Analysis -- RESULTS -- Growth Responses of Bt Cotton and Non-Bt Cotton to SOIL P Levels -- Growth Responses of Bt Cotton and Non-Bt Cotton to Soil Cd Levels -- Growth Responses of Bt Cotton and Non-Bt Cotton to Soil Cu Levels -- DISCUSSION -- ACKNOWLEDGMENTS -- REFERENCES -- THE APPLICATION OF SITE-SPECIFIC RECOMBINATION SYSTEMS FOR BIOSAFETY AND GENOME MANIPULATION IN THE PRODUCTION OF TRANSGENIC PLANTS -- ABSTRACT -- CONVENTIONAL BREEDING AND THE BENEFITS OF TRANSGENIC CROP PRODUCTION -- PRODUCTION OF TRANSGENIC PLANTS -- 1. Vector Construction -- 2. Genetic Transformation of Plants -- 3. Selection of Transgenic Plants -- CONCERNS ABOUT THE USE OF SELECTABLE MARKER GENES -- STRATEGIES FOR PRODUCING MARKER-GENE-FREE TRANSGENIC PLANTS. THE SOURCES OF SITE-SPECIFIC RECOMBINATION SYSTEMS -- MODES OF ACTION OF SITE-SPECIFIC RECOMBINATION -- A BRIEF HISTORY OF THE STUDY OF THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM AND ITS APPLICATION IN EUKARYOTIC DNA EXCISION -- APPLICATIONS OF SITE-SPECIFIC RECOMBINATION SYSTEMS FOR PLANT GENOME MANIPULATION -- 1. Site-Specific Recombination Systems Are Used to Delete Selectable Marker Genes in Plants -- 1.1. Constitutively Expressing a Recombinase Gene for Selectable-Marker-Gene Excision -- 1.2. Transiently Expressing a Recombinase Gene for Selectable-Marker-Gene Excision -- 1.3. Induced Expression of a Recombinase Gene for Selectable-Marker-Gene Excision -- 1.4. Genetically Programmed Recombinase Gene Expression for Selectable Marker Excision -- 2. Site-Specific Recombination Systems Are Used for Resolution-Based Single-Copy Transgenic Line Production -- 3. Site-Specific Recombination Systems Are Used in Breeding Programs for Hybrid Seed Production -- 4. Novel Site-Specific Recombination Systems Can Potentially Be Exploited for Plant Genome Manipulations -- FINAL REMARKS AND FUTURE PERSPECTIVES -- REFERENCES -- AMPLIFICATION OF SMALL INTERFERING RNAS IN TRANSGENIC PLANTS -- ABSTRACT -- INTRODUCTION -- 1. OUTLINE OF RNAI AND RELATED SILENCING PATHWAYS -- 2. PHENOTYPIC VARIATION IN PLANTS SHOWING RNA SILENCING -- 3. CAVEAT TO SIRNA-MEDIATED SILENCING -- 4. GENERATION OF SECONDARY SIRNAS IN TRANSGENIC PLANTS -- 5. RDR6-DEPENDENT TRANSITIVE RNA SILENCING -- 6. TRANSGENE TRANSCRIPTS: PREFERABLE TEMPLATES FOR RDR6 -- 7. POSSIBLE INVOLVEMENT OF NUCLEAR RNA SILENCING PATHWAY DURING TRANSITIVITY -- 8. CASE STUDY: 24-NT SIRNAS PRODUCED FROM AN INTRON-CONTAINING TRANSGENE -- CONCLUSION -- REFERENCES -- INDEX.