Advances in Recovering Plasmids from Wastewater : a State of the Science.
- 1st ed.
- 1 online resource (50 pages)
- WERF Research Report Series ; v.NTRY8R15a .
- WERF Research Report Series .
Cover -- Copyright -- Acknowledgments -- Abstract and Benefits -- Table of Contents -- List of Tables -- List of Figures -- List of Acronyms -- Executive Summary -- Chapter 1.0: Introduction to Plasmids -- 1.1 Introduction -- 1.2 Overview of Plasmids -- 1.2.1 Transfer of Genetic Information -- 1.2.2 Provide the Cellular Machinery for Conjugation -- 1.2.3 Provide Resistance -- 1.2.4 Provide Bacteria a Competitive Advantage -- 1.3 Diversity of Plasmids in Wastewater -- 1.3.1 Tools for the Characterization of Plasmid Diversity in Wastewater -- 1.3.2 Overview on the Diversity of Plasmids in Wastewater -- 1.3.3 Diversity of Plasmid Hosts in WRRFs -- 1.3.4 Wastewater-Sourced Plasmid Genes with Potential Value -- 1.4 Fate and Persistence of Plasmids in Wastewater Treatment Processes -- 1.4.1 Plasmid Quantification -- 1.4.2 Persistence of Plasmids Throughout Treatment Processes -- 1.4.3 Plasmid Transfer Rates in Wastewater Treatment -- 1.4.4 Summary of Plasmid Fate and Persistence in WRRFs -- Chapter 2.0: Mining Plasmids and Their Encoded Information From Wastewater -- 2.1 Introduction -- 2.2 Plasmid Extraction -- 2.3 Culture-Based Gene Functional Screening -- 2.4 Sequence-Based Bioinformatic Functional Screening -- 2.4.1 Plasmid Sequencing -- 2.4.2 Processing -- Chapter 3.0: Plasmid Market Potential -- 3.1 Introduction -- 3.2 Plasmid-Based Products and Their Market Potential -- 3.2.1 Bulk Plasmids with Unknown Gene Function -- 3.2.2 Plasmids with Known Function and No Use Restrictions -- 3.2.3 Plasmids with Known Function and Use Restrictions -- 3.2.4 Intellectual Property and Rights to Use Plasmids -- 3.2.5 Plasmid-Based End Products -- 3.3 The Biotechnology Market Forecast -- 3.4 The Role of Intellectual Property -- 3.5 Evaluating the Potential Revenues for WRRF Plasmid Recovery. Chapter 4.0: Summary of the Risks and Opportunities for WRRFs Interested in Plasmid Recovery -- References.