DNA Methods in Food Safety : Molecular Typing of Foodborne and Waterborne Bacterial Pathogens.

Intro -- Title page -- Copyright page -- List of Contributors -- Preface -- Section I: Typing Method, Analysis, and Applications -- 1 Polymerase Chain Reaction-Based Subtyping Methods -- Randomly amplified polymorphic DNA -- Amplified fragment length polymorphism (AFLP) -- Repetitive-sequence-based PCR -- Multiple-locus variable-number tandem repeat analysis -- PCR-restriction fragment length polymorphism (PCR-RFLP) -- PCR melting profile analysis -- References -- 2 Pulsed-Field Gel Electrophoresis and the Molecular Epidemiology of Foodborne Pathogens -- Background -- Theory -- Parameters critical to PFGE separations -- Instrumentation -- Epidemiological applications of PFGE -- Conclusion -- Acknowledgments -- References -- 3 Multilocus Sequence Typing: An Adaptable Tool for Understanding the Global Epidemiology of Bacterial Pathogens -- Multilocus sequence typing -- MLST databases -- Advantages of MLST -- Types of MLST schemes -- Discriminatory power and epidemiologic concordance -- Clonal complexes, epidemic clones, and outbreak clones -- Conclusions -- Acknowledgments -- References -- 4 High-Throughput Sequencing -- Introduction -- Existing subtyping methods -- WGS: A comprehensive platform for molecular subtyping -- MLST versus WGS -- SNP analysis versus WGS -- Hypervariable markers -- Phenotypic markers versus WGS -- Technical process of WGS -- Computational tools for WGS analysis -- WGS in recent foodborne outbreak investigations -- Challenges and future prospects of WGS in molecular subtyping -- References -- 5 Analysis of Typing Results -- Introduction -- Measuring similarity -- Creating groupings of related isolates -- Comparison of typing methods -- References -- 6 Databases and Internet Applications -- Introduction -- Existing online networks and resources -- Current challenges, possible solutions, and future trends.

GeoGenomic identification and an integrated Web-based global infrastructure -- References -- 7 The Transformation of Disease Surveillance, Outbreak Detection, and Regulatory Response by Molecular Epidemiology -- Epidemiology and surveillance -- Outbreaks -- PulseNet -- From steps to principles -- The end of the culture era? -- Summary -- Acknowledgments -- References -- Section II: Pathogens -- 8 The Genus Bacillus -- Bacillus: A highly heterogeneous genus challenging food quality and safety -- Bacillus toxins are gaining increasing prominence as causative agents of foodborne diseases -- Polymerase chain reaction methods -- Multilocus sequence typing (MLST) and amplified fragment length polymorphism (AFLP): The "golden standards" for population analysis of the B. cereus group -- Pulsed-field gel electrophoresis -- Microarrays and high-throughput sequencing: From genotyping to genomotyping -- Conclusions and future direction -- References -- 9 Molecular Typing of Campylobacter jejuni -- Introduction -- Brief history of typing methods to study C. jejuni -- Most common methods to type C. jejuni -- Less commonly used typing methods -- Comparative genomic fingerprinting -- Combination of techniques -- References -- 10 DNA Typing Methods for Members of the Cronobacter Genus -- Introduction -- Cronobacter pathogenicity and virulence -- Taxonomy and genomic diversity of the Cronobacter genus -- Cronobacter and the food industry -- Biotyping of Cronobacter strains -- DNA-based typing of Cronobacter strains -- Multilocus sequence typing of Cronobacter spp. -- Case studies of using DNA sequence-based typing of Cronobacter spp. -- Current issues in the application of DNA typing methods for Cronobacter spp. -- Conclusions -- Acknowledgments -- References -- 11 Molecular Subtyping Approaches for Pathogenic Clostridium spp. Isolated from Foods -- Introduction.

Concluding remarks -- Disclaimer -- References -- 12 Molecular Characterization of Shiga Toxin-Producing Escherichia coli -- Introduction -- DNA fingerprinting -- Sequence-based genotyping -- Virotyping -- Conclusions -- References -- 13 Molecular Subtyping Methods for Listeria monocytogenes: Tools for Tracking and Control -- Introduction -- Fragment-based methods -- Hybridization-based methods -- DNA sequence-based subtyping methods -- Acknowledgments -- References -- 14 Salmonella -- Introduction -- Restriction analysis-based genotyping -- PCR-based typing methods -- DNA sequencing-based typing methods -- Comparison of molecular subtyping methods for Salmonella -- Conclusions -- Disclaimer -- References -- 15 Vibrio cholerae -- Introduction -- CTX Phage -- CTXcla (classical type CTX) and CTX-1 (El Tor type CTX or CTXEl Tor) -- CTX-2 -- CTX-3 -- ctxB Typing -- TLC element -- Genotyping of V. cholerae -- MLVA analysis of V. cholerae O1 strains -- Conclusions -- References -- Index -- End User License Agreement.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.