Chromatography : Principles and Instrumentation.

Cover -- Title Page -- Copyright -- Contents -- Preface -- Chapter 1 Fundamentals of Chromatography -- 1.1 Theory -- 1.1.1 Component Separation -- 1.1.2 Retention Factor -- 1.1.3 Separation -- 1.1.4 Resolution and Theoretical Plates -- 1.2 Band Broadening -- 1.2.1 Diffusion -- 1.2.2 Linear Velocity -- 1.2.3 Broadening in Open Tubes with No Stationary Phase and No Retention -- 1.2.4 Broadening in Open Tubes with a Stationary Phase -- 1.2.5 Broadening in a Packed Column -- 1.2.6 Putting It All Together -- 1.2.7 Practical Consequences of Broadening Theory -- 1.3 General Resolution Equation -- 1.4 Peak Symmetry -- 1.5 Key Operating Variables -- 1.6 Instrumentation -- 1.7 Practice of The Technique -- 1.7.1 Quantitation -- 1.7.2 Internal Standards and the Method of Standard Additions -- 1.8 Emerging Trends and Applications -- 1.9 Summary -- Problems -- References -- Further Reading -- Chapter 2 Gas Chromatography -- 2.1 Theory of Gas Chromatographic Separations -- 2.1.1 GC Columns and Partitioning -- 2.2 Key Operating Variables that Control Retention -- 2.2.1 Adjusting Retention Time: Temperature -- 2.2.2 Adjusting Retention Time: Temperature Programming -- 2.2.3 Adjusting Retention Time: Mobile Phase Flow Rate -- 2.2.4 Adjusting Retention Time: The Column and the Stationary Phase -- 2.2.5 Adjusting Retention Time: Summary -- 2.2.6 Measures of Retention -- 2.3 Gas Chromatography Instrumentation -- 2.3.1 Carrier Gas Supply -- 2.3.2 The Injection Port and the Solute Injection Process -- 2.3.3 Oven/Column Compartment -- 2.3.4 Detectors -- 2.4 A More Detailed Look at Stationary Phase Chemistry: Kovats Indices and Mcreynolds Constants -- 2.4.1 Kovats Retention Indices -- 2.4.2 Stationary Phase Selection -- 2.5 Gas Chromatography in Practice -- 2.5.1 Syringe Washing -- 2.5.2 Controls and Blanks/Ghost Peaks -- 2.5.3 Autosamplers -- 2.5.4 GC Septa.

2.5.5 Qualitative Analysis -- 2.5.6 Quantitative Analysis -- 2.5.7 Derivatization -- 2.5.8 High-Speed GC -- 2.5.9 Tandem GC -- 2.5.10 Microfabricated GC -- 2.6 A "Real-World" Application of Gas Chromatography -- 2.6.1 GC and International Oil Trading -- 2.7 Summary -- Problems -- References -- Further Reading -- Chapter 3 Liquid Chromatography -- 3.1 Examples of Liquid Chromatography Analyses -- 3.2 Scope of Liquid Chromatography -- 3.3 History of LC -- 3.3.1 Modern Packing Materials -- 3.4 Modes of Liquid Chromatography -- 3.4.1 Normal Phase Liquid Chromatography (NPLC) -- 3.4.2 Reversed-Phase Liquid Chromatography (RPLC) -- 3.4.3 Ion-Exchange Chromatography (IEX) -- 3.4.4 Hydrophilic Interaction Chromatography (HILIC) -- 3.4.5 Size Exclusion Chromatography (SEC) -- 3.4.6 Affinity Chromatography -- 3.5 HPLC Instrumentation -- 3.5.1 The Proportioning Valve -- 3.5.2 Mixing Chamber -- 3.5.3 Pumps -- 3.5.4 Injection -- 3.5.5 The Column and Particles -- 3.5.6 Guard Columns -- 3.5.7 Detectors -- 3.6 Specific Uses of and Advances in Liquid Chromatography -- 3.6.1 Chiral Separations -- 3.6.2 Preparative-Scale Chromatography -- 3.6.3 Ultra-High Performance Liquid Chromatography (UHPLC) for High-Speed Separations -- 3.6.4 Tandem-Column Liquid Chromatography -- 3.6.5 Two-Dimensional Liquid Chromatography (2D-LC) -- 3.7 Application of LC - Analysis of Pharmaceutical Compounds in Groundwater -- 3.7.1 Sampling -- 3.7.2 Analysis Method for 21 Antibiotics - Sample Pretreatment -- 3.7.3 Use of Internal Standards and Other Quality Assurance Issues -- 3.7.4 LC Analyses -- 3.7.5 Mass Spectrometric Selected Ion Monitoring Detection -- 3.7.6 Results -- 3.8 Summary -- Problems -- References -- Solutions -- Index -- EULA.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.