Applications of Molecular Microbiological Methods.

Intro -- Contents -- Contributors -- Foreword -- Introduction -- Overview of chapters -- Part I: Industrial Microbiology -- 1: Molecular Methods in Microbiologically Influenced Corrosion Research, Monitoring and Control -- Introduction -- PCR-based techniques -- Hybridization techniques -- Novel approaches -- Conclusion and perspectives -- 2: Using the Power of Molecular Microbiological Methods in Oilfield Corrosion Management -- Background -- Methods -- Results -- Conclusions and perspectives -- 3: Next-generation Sequencing Approach to Understand Pipeline Biocorrosion -- Introduction -- Molecular methods -- Investigation of MIC -- Case study -- 4: Molecular Microbiological Methods Applied on Ship Ballast Tank Samples -- Introduction -- Case -- Experimental set-up -- Results -- Electrochemical data -- Discussion -- 5: Molecular Characterization of Microbial Communities Associated with ALWC -- Introduction -- Material and methods -- Results and discussion -- Conclusions towards a further understanding of ALWC -- 6: Application of the qPCR Technique for SRB Quantification in the Oil and Gas Industries -- Background -- Methods -- Results and discussion -- Conclusions and perspectives -- 7: Molecular Microbiological Methods Applied to Microbial Methane Production -- Introduction -- Prospects for enhanced methanogenesis from oil reservoirs, coal beds and shale -- Use of MMMs to elucidate microbial communities and activities in oil reservoirs, coal beds and shale -- Case study 1: the use of MMM to pinpoint the key microbial players involved in biodegrading crude oil hydrocarbons in a high-temperature crude oil reservoir -- Case study 2: the use of MMM to characterize the microbial diversity and methanogenic potential of coal seams in the Western Canadian Sedimentary Basin -- Part II: Medical Microbiology.

8: Characterization of Bacterial Communities in Suspected Prosthetic Joint Infections -- Introduction -- Material and methods -- Results -- Discussion -- Conclusion -- 9: Using the Core and Supra Genomes to Determine Diversity and Natural Proclivities -- Definition of species in post-genomic era -- Genomic diversity within bacterial species -- Sources of genomic variability: horizontal gene transfer and point mutations -- The supragenome provides a framework for understanding intra-species bacterial diversity in the post-genomic era -- Mathematical models to represent supragenomes -- Environmental conditions that support HGT -- Conclusion -- Part III: Environmental Microbiology -- 10: Quantitative PCR and Reverse Transcription Quantitative PCR Applied to Methane-cycling Archaea -- Introduction -- Methods -- Results -- Discussion -- 11: The Metabolic Function of Uncultured Microorganisms -- Introduction -- Methods for studying single cells -- Case study 1: discovery of novel carbon fixing microorganisms by genomic analyses of single cells -- Untangling the genomic information from a single cell -- Studying physiological capabilities in single microbial cells -- Conclusion and outlook -- 12: Assessing Microbial Activity and Degradation Pathways in the Environment -- Introduction -- Conclusions -- Part IV: Applied Molecular Microbiological Methods -- 13: Metagenomic Analysis of Microbial Communities and Beyond -- Introduction -- Target enrichment -- DNA extraction -- Sequencing -- Sequence assembly -- Gene prediction and annotation -- Targeted metagenomics -- The function-driven metagenomic approach -- Beyond metagenomics - meta-analysis of expression data -- Concluding remarks -- 14: Stable Isotope Probing in Environmental Microbiology Studies -- Introduction -- Methodologies -- Summary.

15: FISH for the Identification and Quantification of Microorganisms -- Method basics -- Pros and cons of rRNA FISH -- Technology applicability -- 16: Quantitative Real-time Polymerase Chain Reaction Methods for Abundance and Activity Measures -- Introduction -- Fluorescence measurements - the heart of qPCR -- Abundance measurements -- Activity measurements -- Technology applications -- Technology considerations -- 17: Investigation of Microorganisms at the Single-cell Level -- Introduction -- Raman microspectroscopy -- High-resolution secondary ion mass spectrometry (SIMS) -- Advantages and disadvantages of Raman microspectroscopy and high-resolution SIMS -- Index -- Current books of interest.

This book, written by international experts in the field, presents emerging molecular methods that allow the diversity of a microbial community to be surveyed and its functions to be investigated. The first part of the book provides examples of the application of molecular microbiological methods in various industrial applications. Part two deals with the identification of microorganisms in medical settings while the third part presents case studies that use molecular methods to assess the structure and function of microbial communities in natural environments. The fourth part of the book describes in greater detail the methods and technologies featured in the preceding case studies including metagenomics, stable isotope probing, fluorescence in situ hybridization, quantitative PCR, reverse transcription PCR and single cell methods. These detailed descriptions enable readers to evaluate the applicability of various tools for approaching questions and case studies of their own. This practical, authoritative and up-to-date volume is a valuable resource for anyone applying or developing molecular methods in any area of microbiology and is a recommended acquisition for all microbiology laboratories.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.