Cyanobacteria : Omics and Manipulation.

Intro -- Contents -- Preface -- Current Books of Interest -- 1 The Cyanobacterial Core Genome: Global and Specific Features with a Focus on Secondary Metabolites -- Introduction -- Metagenome analysis and information in cyanobacterial research -- Recent advances in pangenome assignment -- Detection of core- and clade-specific functions via metagenome analysis -- General principles of secondary metabolite biosynthesis in cyanobacteria -- Main classes of secondary metabolites in cyanobacteria -- Secondary metabolite gene clusters in the cyanobacterial pangenome -- Conclusions -- Future trends and challenges -- 2 Genome-wide Analysis of Cyanobacterial Evolution: The Example of Synechococcus -- Introduction -- The origin of Synechococcus -- The genome and pan-genome of cyanobacteria -- Speciation in cyanobacteria -- Genome-scale reconstruction of evolutionary relationships: phylogenomics -- Conclusion -- Future trends -- 3 Genomics of NRPS/PKS Biosynthetic Gene Clusters in Cyanobacteria -- Introduction -- Genome mining for NRPS/PKS biosynthetic gene clusters -- Comparative genomics of biosynthetic gene clusters -- Biosynthetic gene clusters conservation -- From prediction to characterization -- Genomics from isolated strains versus metagenomics -- Conclusion -- Future trends -- 4 RNA-seq Based Transcriptomic Analysis of Single Cyanobacterial Cells -- Introduction -- Quantitation of total RNA in single Synechocystis cells -- Single-cell RNA isolation -- Whole-transcriptome amplification -- Quality control -- RNA-seq analysis of single-cell transcriptome -- Verification of RNA-seq results -- Technical and biological significance -- Conclusions -- Considerations and future trends -- 5 Transcriptomics of Cyanobacterial Stress Responses: Genes, Sensors and Molecular Triggers -- Introduction -- Heat stress and other abiotic stresses.

Cold stress and other abiotic stresses -- Conclusions: Sensors and universal triggers of stress responses -- 6 Transcriptomic and Proteomic Analysis to Understand Systems-level Properties of Diurnal Cycles in Nitrogen-fixing Cyanobacteria -- Introduction -- Systems biology and proteomics -- Cyanobacterial proteomics -- Protein-protein interactions -- Growth of cyanobacteria in the light and dark -- Conclusion -- Future research directions -- 7 Proteomic Analysis of Post Translational Modifications in Cyanobacteria -- Introduction -- PTMs and proteomic analysis of PTMs in cyanobacteria -- Challenge and perspectives in PTM proteomics of cyanobacteria -- Conclusions -- 8 Metabolic Engineering and Systems Biology for Free Fatty Acid Production in Cyanobacteria -- Introduction -- Metabolic engineering of FFA production in cyanobacteria -- Systems biology for FFA production in cyanobacteria -- Conclusions and future trends -- 9 Terpene Hydrocarbons Production in Cyanobacteria -- Introduction: state of the art -- Perspective -- The process -- Solar-to-product energy conversion efficiency and photosynthetic productivity -- Principles of CO2 delivery and terpene hydrocarbons sequestration -- Efficacy of terpene hydrocarbons as biofuels -- Efficacy of alcohol molecules as biofuels -- Conclusion -- 10 Ethanol Production in Cyanobacteria: Impact of Omics of the Model Organism Synechocystis on Yield Enhancement -- Introduction -- Ethanol production in cyanobacteria -- The construction of the pdc/adh cassette -- The origin of the pdc/adh gene cassette -- The Zymomonas mobilis (Zm) pyruvate decarboxylase -- The native ADH of Synechocystis -- The Zymomonas mobilis (Zm) ADH -- The effect of the ADH co-factor -- Some key issues with metabolic engineering of Synechocystis -- Strains and substrains and genomics of the cell factory Synechocystis.

Proteomic analysis in response to ethanol -- Transcriptomic analysis in response to ethanol -- Modelling of metabolic flux in Synechocystis to enhance ethanol production -- Theoretical estimates of ethanol yields -- Towards industrial production of ethanol from cyanobacteria -- Conclusion and future trends -- 11 Engineering of Alkane Production in Cyanobacteria -- Introduction -- Alkane profiles in native cyanobacteria -- Molecular tools for genetic engineering of cyanobacteria -- Systems biology research on cyanobacterial alkanes -- Fatty acid production in engineered cyanobacteria -- Alkane production in engineered cyanobacteria -- Protein engineering for ADO -- Conclusions -- Future trends -- 12 Photoautotrophic Polyhydroxyalkanoate Production in Cyanobacteria -- Introduction -- Cyanobacteria as platforms for biomass production -- Cyanobacteria are native PHA producers -- Engineering cyanobacteria for PHA production -- Future trends -- Index.

Topics covered: The cyanobacterial core-genome with a focus on secondary metabolites; cyanobacterial evolution; genomics of NRPS/PKS biosynthetic gene clusters; RNA-seq based transcriptomic analysis of single cyanobacterial cells; transcriptomics of the responses: genes, sensors, and molecular triggers; transcriptomic and proteomic analysis of diurnal cycles in nitrogen-fixing cyanobacteria; proteomic analysis of post translational modifications; metabolic engineering and systems biology for free fatty acid production; isoprene production; ethanol production: impact of omics of the model organism Synechocystis on yield enhancement; engineering of alkane production; photoautotrophic polyhydroxyalkanoate production. This cutting-edge text will serve as a valuable resource for all those working in this field and is recommended for all microbiology libraries.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.