The CRISPR/Cas System : Emerging Technology and Application.

Intro -- Contents -- Current Books of Interest -- Dedication -- Preface -- 1. Type III CRISPR/Cas System: Introduction and its Application for Genetic Manipulations -- Introduction -- Functions of Type III CRISPR/Cas systems -- Genetic manipulation by Type III CRISPR/Cas system -- Conclusions -- 2. dCas9: A Versatile Tool For Epigenome Editing -- Introduction -- Epigenome editing -- dCas9 design strategies -- Applications of the dCas9 tool -- Conclusions and future perspectives -- Glossary -- 3. Treating Genetic Disorders Using State-of-the-Art Technology -- Introduction -- CRISPR/Cas9 -- Ex vivo genome editing -- In vivo genome editing -- CRISPR/Cas9 to treat movement disorders -- Treating cystic fibrosis -- CRISPR/Cas9 to treat eye and ear diseases -- Editing epigenome to treat cancer -- CRISPR/Cas9 to generate disease models -- Genome editing of viruses -- How to address the challenges associated with the application of CRISPR/Cas9 technology? -- Conclusion and future directions -- 4. An Era of CRISPR/Cas9-mediated Plant Genome Editing -- CRISPR/Cas9: ultimate tool for multiplex genome editing in plants -- Applications in plants -- Harnessing the potential of CRISPR/Cas9 for disease resistance in crops -- Conclusions -- 5. CRISPR/Cas9-mediated Immunity in Plants Against Pathogens -- Introduction -- SA-, JA- and ET-mediated defence regulation against pathogens -- CRISPR/Cas9 role in plant immunity -- Multiplexing of genome editing -- Conclusion -- 6. Improving CRISPR/Cas9 On-target Specificity -- Introduction -- CRISPR off-targets -- Analysing off-target effects -- Genome wide assays for interrogation of off-target sites -- In vitro genome-wide assay for the detection of off-targets sites -- gRNA designing tools -- How to improve the specificity of CRISPR/Cas9 system? -- Lesson learnt from biochemical, structural and functional studies.

Specificity achieved by Cas9 engineering -- Concentration of Cas9-gRNA -- What else should be considered in avoiding off-target mutagenesis? -- Future directions -- Glossary -- 7. CRISPR-mediated Genome Engineering and its Application in Industry -- Introduction -- Application of CRISPR technology to treatment of microbial infection -- Application of CRISPR technology to food and agricultural sciences -- Application of CRISPR technology to energy sciences -- Future direction -- 8. Applications of CRISPR/Cas9 in Reproductive Biology -- Introduction -- Gene drives and CRISPR/Cas9 -- Genome engineering and reproductive biology -- CRISPR/Cas system in male reproduction -- CRISPR/Cas system in female reproduction -- Conclusion -- 9. Ethical Issues Regarding CRISPR-mediated Genome Editing -- Introduction -- Ethical issues regarding CRISPR technology -- Way to go forward … caution! -- Conclusion -- Index.

In this volume expert international authors provide a useful and timely review of the applications of the CRISPR/Cas system across diverse fields and explore further avenues and research directions of this novel and powerful editing technology. The technology and its application are reviewed with respect to reproduction and development, immunity and genetic diseases, system structure and system specificity. Some of the potential problems of the CRISPR/Cas system are also discussed, in particular the specificity of the system: this remains an important topic as improvement could lead to the more direct and efficient use of the CRISPR/Cas system in clinical settings. The authors also debate ethical concerns associated with this powerful new technology. This volume is a rigorous review of the applications and new opportunities for the CRISPR/Cas system and provides a stimulus for current and future research. An invaluable guide for all scientists working in the fields of genome editing and gene therapy the book is also recommended for all life sciences libraries.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.