SUMOylation and Ubiquitination : Current and Emerging Concepts.

Intro -- Contents -- Current Books of Interest -- Preface -- Part I: General Principles -- 1: The Rise of the Ubiquitin Super Family -- Introduction: the history of discovery -- Ubl proteins -- Ubl enzymology -- Ub/SUMO functions -- Ubiquitin-SUMO crosstalk -- Conclusions -- 2: Cracking the Ubiquitin Code: The Ubiquitin Toolbox -- Introduction -- Chemical approaches to ubiquitination -- Visualizing ubiquitin in action - Ub reagents targeting DUBs and ligases -- What does the future hold? -- Conclusion -- 3: Recent Highlights: Onco Viral Exploitation of the SUMO System -- Introduction -- Oncoviruses exploitation of the SUMO pathway -- Conclusions -- 4: Progress in the Discovery of Small Molecule Modulators of DeSUMOylation -- Introduction -- SUMO modification and its cellular functions -- DeSUMOylation and SENPs -- DeSUMOylation in diseases -- Advances in the development of SENP inhibitors -- Part II: Novel and Advancing Technologies -- 5: Identification of SUMOylated and Ubiquitinated Substrates by Mass Spectrometry -- Introduction -- Enrichment methods for large-scale ubiquitin site identification -- Methods for large-scale SUMO site identification -- Current methods to study ubiquitin and SUMO co-modified proteins -- Concluding remarks and future perspectives -- 6: Global Proteomic Profiling of SUMO and Ubiquitin -- The function of SUMO -- Identification of SUMOylated proteins -- Global SUMO profiling approaches -- New discoveries of SUMO functions in biology -- 7: Biotin-based Approaches for the Study of Ubiquitin and Ubiquitin-like Protein Modifications -- Exploiting the biotin-streptavidin interaction for biotechnology -- The complexity of the ubiquitin code -- Fishing for ubiquitomes using bioUb and BirA -- Beyond ubiquitin: expanding the bioUbL toolbox -- Biotin-mediated proximity proteomics for exploring the Ub/UbL network.

Future perspectives -- 8: Screening Mammalian SUMOylated Proteins by Fluorescence Protein Reconstitution -- Introduction -- The whole procedure of the novel screening method based on fluorescence protein reconstitution in mammalian cells -- Identification of SUMOylated proteins -- Conclusion -- 9: Dissecting Complex SUMOylation Networks in Humans -- Complexity of SUMOylation cellular networks -- Protein microarray technology -- Functional protein microarrays -- Comprehensive SUMOylation network construction using HuProt array technology -- Global analysis of SUMO E3 ligase specificity using a protein microarray platform: -- Global SUMOylation results -- Crosstalk between protein phosphorylation and SUMOylation -- Regulation by non-covalent interactions with SUMO -- SUMO binding study using HuProt™ array -- Integration of covalent and non-covalent SUMO networks -- Conclusions -- 10: TULIP: Targets of Ubiquitin Ligases Identified by Proteomics -- Introduction -- TULIP methodology -- Further confirmation of the E3OI targets -- Part III: Cellular Processes -- 11: Regulation of p53 Family Members by the Ubiquitin and SUMO Modification Systems -- Introduction: the p53 family -- The tumour suppressor p53 -- The transcription factor p63 in development and tumorigenesis -- Role of p73 in neurological development and cancer -- Conclusions -- 12: Interplay between the Ubiquitin Proteasome System and Mitochondria for Protein Homeostasis -- Introduction -- Principles of protein quality control - cytosolic and mitochondrial -- Mitochondrial roles in quality control of cytosolic components -- Quality control of mitochondrial proteins in the cytoplasm -- Concluding remarks -- 13: Interplay of Ubiquitination and SUMOylation with miRNAs -- The life as small RNAs -- Ubiquitination -- Sumoylation -- The post-transcriptional and post-translational interactions.

Micro-RNAs and the ubiquitination pathway -- Micro-RNAs and the SUMOylation pathway -- 14: The Role of Ubiquitination and SUMOylation in DNA Replication -- Regulation of eukaryotic DNA replication -- Ubiquitin-dependent regulation of DNA replication -- SUMO-dependent regulation of replication initiation -- Ubiquitin and SUMO regulation of DNA synthesis -- Regulation of replication termination by ubiquitin and SUMO -- Concluding remarks -- 15: Roles of Ubiquitination and SUMOylation in DNA Damage Response -- Introduction -- Overview of the ubiquitin signalling -- Overview of the SUMO signalling -- Ubiquitin and SUMO signalling in HR -- Ubiquitin and SUMO signalling in NHEJ -- Ubiquitin and SUMO signalling in inter-strand crosslink repair (ICLR) -- Ubiquitin and SUMO signalling in nucleotide excision repair (NER) -- Ubiquitin and SUMO signalling in base excision repair (BER) -- Discussion and future perspectives -- 16: The Role of Ubiquitination and SUMOylation in Telomere Biology -- Telomere structure and function -- The Hayflick limit and the end replication problem -- Telomeres and protecting the genome -- T-loops -- The shelterin complex -- Protein turnover -- The Function of the ubiquitin-proteasome system and the process of SUMOylation -- Telomere regulation in cancer -- Telomerase -- Alternative lengthening of telomeres (ALT) -- Ubiquitination-mediated regulation of the shelterin -- SUMOylation-mediated regulation of the shelterin -- Telomere chromatin regulation by ubiquitin -- Discussion and perspectives -- 17: Role of Ubiquitin and SUMO in Intracellular Trafficking -- Posttranslational modifications in regulation of cellular processes -- Ubiquitin and SUMO as signals regulating membrane trafficking and endocytosis -- Ubiquitin and SUMO as signals regulating nucleocytoplasmic shuttling and subnuclear targeting.

The genetic evidence in vivo and the significance of ubiquitin and SUMO mediated regulation of trafficking in human diseases -- Conclusions -- 18: Roles of Ubiquitination and SUMOylation in the Regulation of Angiogenesis -- Introduction -- Molecular basics of angiogenesis -- PTMs in angiogenesis -- Ubiquitination and SUMOylation in angiogenesis -- Conclusions and remarks -- 19: The Role of SUMOylation and Ubiquitination in Brain Ischaemia: Critical Concepts and Clinical Implications -- SUMOylation in brain ischaemia/stroke -- Ubiquitination in brain ischaemia/stroke -- Conclusions -- 20: The Role of Ubiquitination and SUMOylation in Autophagy -- Introduction -- Ubiquitin and autophagy -- SUMOylation and autophagy -- Concluding remarks -- 21: Ubiquitin and SUMO Modifications in Caenorhabditis elegans Stress Response -- Introduction -- Components of ubiquitin pathway in Caenorhabditis elegans -- Components of SUMO pathway in Caenorhabditis elegans -- The universe of proteins modified by ubiquitin and SUMO -- SUMO and ubiquitin in stress response and ROS signalling -- Protein homeostasis and ageing -- Infections and innate immunity -- SUMO in protein complex assembly -- Conclusions -- Part IV: Infection, Immunity and Disease -- 22: Beyond Degradation: Ubiquitination of the Inflammasome Regulates Assembly and Activity -- Inflammasomes -- Ubiquitin -- NLRP3 -- AIM2 -- NLRP7 -- ASC -- IL-1β -- Conclusions -- 23: Ubiquitin and SUMO in Antiviral Defence -- Introduction: immunity, immunity, immunity -- Intrinsic immunity -- Innate immunity -- Acquired immunity -- Conclusions -- 24: Ubiquitination and SUMOylation in HIV Infection: Friends and Foes -- Introduction to HIV and AIDS -- Importance of post-translational modifications for a productive HIV infection -- Relevance of ubiquitination and SUMOylation for a successful infection. Part I: HIV proteins.

Relevance of ubiquitination and SUMOylation for a successful infection. Part II: HIV dependency factors -- Ubiquitination and SUMOylation as a mechanism of innate defence against HIV -- Ubiquitination as a viral countermeasure to restriction factors -- Manipulation of SUMO and ubiquitin cascades as therapeutic strategies against HIV -- Concluding remarks -- 25: Ubiquitination and SUMOylation of Amyloid and Amyloid-like Proteins in Health and Disease -- Introduction -- Toxic amyloids -- Functional amyloid-like proteins. -- Conclusion -- 26: Keeping Up with the Pathogens: The Role of SUMOylation in Plant Immunity -- Introduction -- Immunity -- Small ubiquitin-like modifier (SUMO) -- Phase 1-PTI -- Phase 2-ETS -- Phase 3-ETI -- Phase 4 -- Future work -- Conclusions -- Index -- Blank Page.

Written by highly respected leaders in their fields under the expert guidance of the editor, this volume covers the principles of ubiquitination and SUMOylation, presents detailed reviews of current and emerging concepts and highlights new advances in all areas of SUMOylation and ubiquitination. Topics of note include: the ubiquitin superfamily, the ubiquitin toolbox, onco viral exploitation of the SUMO system, small molecule modulators of desumoylation, mass spectrometry, global proteomic profiling of SUMO and ubiquitin, biotin-based approaches, genetic screening, SUMOylation networks in humans, targets for ubiquitin ligases, regulation of p53, protein homeostasis, miRNAs, DNA replication, DNA damage response, telomere biology, intracellular trafficking, regulation of angiogenesis, brain ischemia, autophagy, assembly and activity, antiviral defense, HIV infection, amyloid and amyloid-like proteins, plant immunity. This comprehensive and up-to-date book is the definitive reference volume on all aspects of SUMOylation and ubiquitination and is an essential acquisition for anyone involved in this area of biology.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.