Real-Time PCR : Advanced Technologies and Applications.

Intro -- Contents -- Contributors -- Preface -- Ch 01: Homogeneous Fluorescent Chemistries for Real-time PCR -- Ch 02: Internal and Other Controls for Real-time PCR Validation -- Ch 03: Analysis of mRNA Expression by Real-time PCR -- Ch 04: Detection of Biothreats by Real-time PCR -- Ch 05: Veterinary Applications of Real-time PCR for Detection and Diagnosis of Infectious Agents -- Ch 06: Applications in Clinical Microbiology -- Ch 07: The Extraction and Purification of Nucleic Acids for Analysis by PCR -- Ch 08: Oligonucleotide Primers and Probes: Use of Chemical Modifications to Increase or Decrease the Specificity of qPCR -- Ch 09: Real-time PCR Arrays -- Ch 10: The Validation of Real-time PCR Assays for Infectious Diseases -- Ch 11: MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay -- Ch 12: Management Aspects of Real-time PCR-based Assay Development, Validation,Verification and Implementation -- Index.

This essential manual provides both the novice and experienced user with an invaluable reference to a wide-range of real-time PCR technologies and applications and provides an overview of the theory of this increasingly important technique. Renowned international authors present detailed technical insights into the underlying principles, methods and practice of real-time PCR. The initial chapters cover the important aspects of real-time PCR including choosing an instrument and probe system, set-up, nucleic acid synthesis, sample extraction controls, and validation and data analysis. Further chapters provide a comprehensive overview of important real-time PCR methodologies such as quantification, expression analysis and mutation detection. This is complemented by the final chapters, which address the application of real-time PCR to diagnosis of infectious diseases, biodefence, veterinary science, food authenticity and molecular haplotyping. This timely and authoritative volume serves both as a basic introduction to real-time PCR and as a source of current trends and applications for those already familiar with the technology. The editors also aim to stimulate readers of all levels to develop their own innovative approaches to real-time PCR. An essential book for all laboratories using PCR.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.