Lactobacillus Genomics and Metabolic Engineering.

Intro -- Contents -- Current Books of Interest -- Preface -- 1. A Genomic Perspective on Niche Adaptability in Lactobacillus -- Introduction -- Genomics of Lactobacillus and insights into niche adaptation -- The dairy niche -- Other food-related niches -- The plant niche -- The human gastrointestinal tract as an environmental niche -- The animal gastrointestinal tract as an environmental niche -- The vaginal niche -- Concluding remarks -- 2. Genetics and Genomics of Lactobacillus sakei and Lactobacillus curvatus -- Introduction: generalities of lactic acid bacteria -- Lactobacillus sakei clade -- Genomic context -- L. sakei and L. curvatus in meat products -- L. sakei and L. curvatus, as ubiquitous species -- Bacteriocins -- Biogenic amines -- Conclusions -- 3. Complex Oligosaccharide Utilization Pathways in Lactobacillus -- Introduction -- Fructan and fructooligosaccharide catabolic pathways -- Metabolism of glucans and glucooligosaccharides -- β-Glucan metabolic pathways in Lactobacillus -- Metabolism of xylooligosaccharides -- Metabolism of galactooligosaccharides -- Metabolism of human milk oligosaccharides (HMOs) -- Concluding remarks -- 4. Production of Lactate Using Lactobacillus -- Introduction -- Lactobacillus genus -- Metabolism of sugar by Lactobacillus -- Lactic acid -- Polylactic acid: PLA -- Problems and solutions in the formation of lactate by fermentation process with Lactobacillus species -- Production of optically pure stereoisomer -- Acid tolerance and decrease in by-product formation -- Use of substrates from industrial waste -- Prevention of phage infection -- An example of lactate production with Lactobacillus casei in cheese whey supplement with different alternatives nitrogen sources -- Concluding remarks -- 5. Modifications of Lactobacillus Surface Under Environmental Stress Conditions -- Introduction.

Lactic acid bacteria -- Starter cultures -- Cell envelopes -- Peptidoglycan: chemical composition -- Penicillin-binding proteins (PBP) -- Teichoic acids -- S-layer -- Wall polysaccharides -- Osmotic stress -- Variations in membrane compositions under osmotic stress -- Variations in the cell wall composition under osmotic stress -- Variation in S-layer protein under environmental stress conditions -- Concluding remarks -- S-Layer Proteins from Lactobacilli: Biogenesis, Structure, Functionality and Biotechnological Applications -- Introduction -- Occurrence and primary structure of Lactobacillus S-layer proteins -- Posttranslational modifications in Lactobacillus S-layer proteins: glycosylation -- Expression of Lactobacillus S-layer protein genes -- Cell wall binding and self-assembly regions in Lactobacillus S-layer proteins -- Secondary and tertiary structure of Lactobacillus S-layer proteins -- Self-assembly of Lactobacillus S-layer proteins in solution -- Functionality of S-layer proteins on Lactobacillus cells -- Biotechnological applications of Lactobacillus S-layer proteins -- Conclusions and future trends -- 7. Bacteriophages of Lactobacillus Species -- Introduction -- Genomics -- Prophages and the risk of lysogenic strains -- Phage-host interaction -- Future trends -- Concluding remarks -- 8. DNA Transfer in Lactobacillus: An Overview -- Introduction -- Natural gene transfer systems: transformation, transduction and conjugation -- Artificial means of DNA transfer: protoplast and electroporation -- Concluding remarks -- Future trends -- 9. Recombinant Gene Expression in Lactobacilli: Strategies and Applications -- Introduction -- Plasmid based protein expression in lactobacilli -- Inducible gene expression -- Constitutive gene expression -- Chromosome based heterologous gene expression.

Additional factors influencing expression strength: 5′-untranslated region, Shine-Dalgarno sequence and codon usage -- Secretory expression of target proteins -- Surface display in lactobacilli -- Therapeutic applications of lactobacilli -- 10. Genomic Overview of Acquired Antibiotic Resistance Mechanisms in Lactobacillus -- Introduction -- Antibiotic resistance mechanisms in bacteria -- Bioinformatic approach to detect antibiotic resistance genes in Lactobacillus genomes -- Conclusions -- Index.

The considerable commercial importance has stimulated a plethora of research projects studying the genomics, molecular biology and metabolic engineering of these bacteria and prompted the need for this concise book which provides a timely overview of the field. Topics covered include: the genomic perspective on niche adaptability; catabolic pathways of complex carbohydrates metabolism; the production of lactate; genetics, biology of the cell envelope and applications of the S-layer proteins; lactobacilli bacteriophage; DNA transfer into Lactobacillus species; recombinant gene expression and genomics of antibiotic resistance in Lactobacillus. This book is an invaluable source of information and essential reading for everyone working with lactobacilli, lactic acid bacteria and probiotics, from the PhD student to the experienced scientist, in academia, the pharmaceutical or biotechnology industries and those working in clinical environments.

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Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.