An Introduction to Fluorescence Correlation Spectroscopy. (Record no. 36811)
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| 001 - CONTROL NUMBER | |
| control field | EBC31253159 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MiAaPQ |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240724115947.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
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| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr cnu|||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240724s2021 xx o ||||0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780750320801 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 9780750320818 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (MiAaPQ)EBC31253159 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (Au-PeEL)EBL31253159 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)1429737102 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MiAaPQ |
| Language of cataloging | eng |
| Description conventions | rda |
| -- | pn |
| Transcribing agency | MiAaPQ |
| Modifying agency | MiAaPQ |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QD96.F56 W645 2020 |
| 082 0# - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 543.56 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Wohland, Thorsten. |
| 245 13 - TITLE STATEMENT | |
| Title | An Introduction to Fluorescence Correlation Spectroscopy. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Bristol : |
| Name of producer, publisher, distributor, manufacturer | Institute of Physics Publishing, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2021. |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | ©2020. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (368 pages) |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 490 1# - SERIES STATEMENT | |
| Series statement | Biophysical Society-IOP Series |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Intro -- Preface -- Acknowledgements -- About the authors -- Thorsten Wohland -- Sudipta Maiti -- Radek Macháň -- Chapter 1 Introduction -- 1.1 What is fluorescence correlation spectroscopy all about? -- 1.2 What do 'fluorescence', 'correlation' and 'spectroscopy' have to do with measuring change? -- 1.3 What can FCS do for you? -- 1.4 What does an FCS measurement involve? -- 1.5 A brief history of FCS -- 1.5.1 Early work -- 1.5.2 The year of the invention -- 1.5.3 The initial progress -- 1.6 Critical technical steps of the revolution -- 1.6.1 Fluorescence: towards single molecule sensitivity -- 1.6.2 Microscopic volume -- 1.6.3 The confocal technique -- 1.6.4 Modern detectors -- 1.6.5 The data processors -- 1.7 Where is FCS now? -- References -- Chapter 2 Correlation functions -- 2.1 Introduction -- 2.2 Fluctuations -- 2.3 Correlations -- 2.4 From correlation coefficient to correlation function -- 2.5 The autocorrelation function (ACF) and its properties -- 2.6 The cross-correlation function (CCF) and its properties -- 2.7 Fluctuations and correlations -- 2.8 Synopsis -- 2.9 Exercises -- References -- Chapter 3 Fluorescence excitation and detection -- 3.1 The probe volume in FCS -- 3.1.1 Introduction -- 3.1.2 The significance of the size of the probe volume -- 3.1.3 A brief introduction to the generation of the fluorescence signal -- 3.1.4 Optical designs applied to obtain an appropriate probe volume for FCS -- 3.2 Photon detection -- 3.2.1 Photon counting -- 3.2.2 Array detectors -- 3.2.3 Photomultiplier tubes -- 3.3 Exercises -- References -- Chapter 4 Data structure, correlation and processing -- 4.1 Software correlators -- 4.1.1 Binned intensity trace-linear correlator -- 4.1.2 Binned intensity trace-multiple-tau correlator -- 4.1.3 Time-tagged intensity trace -- 4.1.4 Cross-correlation calculation and correlation function amplitude. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 4.1.5 Correlation function calculation via Fourier transform -- 4.2 Hardware correlators and their comparison with software correlators -- 4.3 Temporal resolution of correlation functions -- 4.4 Statistical filtering in correlation function calculation -- 4.4.1 Fluorescence lifetime and its integration into FCS datasets -- 4.4.2 Generation of statistical filters in fluorescence lifetime correlation spectroscopy (FLCS) -- 4.4.3 Generalisation of the FLCS principle -fluorescence spectral correlation spectroscopy (FSCS) and filtered FCS (fFCS) -- 4.5 Synopsis -- 4.6 Exercises -- References -- Chapter 5 Theoretical FCS models -- 5.1 The autocorrelation function for diffusion -- 5.2 General characteristics of the ACF for diffusion -- 5.3 Including multiple particles -- 5.4 Anomalous diffusion -- 5.5 Flow -- 5.6 Including multiple processes -- 5.7 Spatial and spatiotemporal correlation techniques -- 5.7.1 Two-focus FCS -- 5.7.2 Scanning FCS -- 5.7.3 Circular scanning fluorescence correlation spectroscopy -- 5.7.4 Image correlation spectroscopy (ICS) -- 5.7.5 Spatiotemporal image correlation spectroscopy (STICS) -- 5.7.6 Raster image correlation spectroscopy (RICS) -- 5.7.7 Imaging fluorescence correlation spectroscopy (Imaging FCS) -- 5.7.8 The FCS diffusion law -- 5.8 Other FCS modalities -- 5.9 Synopsis -- 5.10 Exercises -- References -- Chapter 6 Theoretical fluorescence cross-correlation spectroscopy (FCCS) models -- 6.1 Introduction -- 6.2 Dual-colour FCCS (DC-FCCS) -- 6.2.1 Cross-correlation amount -- 6.2.2 Unequal and non-aligned observation volumes -- 6.2.3 Spectral crosstalk -- 6.2.4 Non-correlated background -- 6.2.5 Non-fluorescent binding partners and free fluorophores -- 6.2.6 Fluorescence quenching and Förster resonance energy transfer (FRET) between fluorophores a and b -- 6.2.7 Complex stoichiometry. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 6.3 FCCS modalities derived from DC-FCCS -- 6.3.1 Single-wavelength FCCS (sw-FCCS) -- 6.3.2 FCCS with alternating laser excitation -- 6.4 Statistical filtering in FCCS -- 6.4.1 Statistical filtering and sources of DC-FCCS artefacts -- 6.4.2 Statistical filtering and negative CCF amplitudes -- 6.4.3 Quasi pulsed interleaved excitation FCCS (PIE-FCCS) -- 6.4.4 Reaction kinetics studied by FCCS -- 6.5 Synopsis -- 6.6 Exercises -- References -- Chapter 7 Artefacts in FCS -- 7.1 Background -- 7.2 Rare events -- 7.3 Bleaching -- 7.4 Sample movement -- 7.5 Detector-related artefacts: after-pulsing and dead time -- 7.6 Detector saturation -- 7.7 Fluorophore saturation -- 7.8 Scattering -- 7.9 Autofluorescence -- 7.10 Sample topology -- 7.11 Immobile particles -- 7.12 Refractive index mismatch -- 7.13 Exercises -- References -- Chapter 8 Data fitting -- 8.1 Introduction -- 8.2 What do we minimize? -- 8.3 The data structure and bias in FCS -- 8.3.1 The data structure in FCS -- 8.3.2 The bias of correlation functions -- 8.4 The standard deviation in FCS -- 8.4.1 Koppel's standard deviation and its modifications -- 8.4.2 Standard deviations from multiple measurements -- 8.4.3 Standard deviation derived from the intensity trace -- 8.4.4 Standard deviation and bias within the ACF -- 8.4.5 The problem of correlations within the ACF -- 8.5 Non-linear least squares fit -- 8.5.1 Least squares and the χ2 function -- 8.5.2 The Levenberg-Marquardt fitting algorithm -- 8.6 Generalized least squares fit -- 8.6.1 The covariance matrix for the ACF -- 8.6.2 Regularization -- 8.7 Global fit -- 8.8 Maximum entropy method -- 8.9 Pairwise model selection using the F-test -- 8.10 Bayes model selection -- 8.11 Practical aspects -- 8.12 Synopsis -- 8.13 Exercises -- References -- Chapter 9 FCS and FCCS measurement strategies -- 9.1 Measuring concentrations by FCS. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 9.1.1 Concentration range accessible by FCS -- 9.1.2 Effective observation volume calibration -- 9.1.3 Molecular brightness determined by FCS -- 9.2 Characterising molecular diffusion by FCS -- 9.2.1 Confocal observation volume radius calibration -- 9.2.2 Calibration-free FCS modalities -- 9.2.3 Imaging FCS and its calibration -- 9.2.4 Separation of multiple processes contributing to the ACF temporal decay -- 9.2.5 Diffusion in two-dimensional systems studied by FCS -- 9.3 Molecular interactions studies by FCS -- 9.3.1 Using changes in the ACF amplitude -- 9.3.2 Using changes in the ACF temporal decay -- 9.4 Molecular interactions studies by FCCS -- 9.4.1 The importance of relating the CCF amplitude to ACF amplitudes -- 9.4.2 Determination of the FCCS experiment dynamic range -- 9.5 Synopsis -- 9.6 Exercises -- References -- Chapter 10 Where to go from here? -- 10.1 Introduction -- 10.2 What FCS can and cannot do -- 10.3 Data acquisition -- 10.4 Data analysis -- 10.5 Related techniques -- 10.6 Some final remarks -- References -- Chapter -- Chapter 2 -- Chapter 3 -- Chapter 4 -- Chapter 5 -- Chapter 6 -- Chapter 7 -- Chapter 8 -- Chapter 9. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | A comprehensive course on fluorescence correlation spectroscopy covering an explanation of basic concepts, derivation of theoretical models, and experimental realisation of the technique. |
| 588 ## - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Description based on publisher supplied metadata and other sources. |
| 590 ## - LOCAL NOTE (RLIN) | |
| Local note | Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Fluorescence spectroscopy. |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Maiti, Sudipta. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Macháň, Radek. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Main entry heading | Wohland, Thorsten |
| Title | An Introduction to Fluorescence Correlation Spectroscopy |
| Place, publisher, and date of publication | Bristol : Institute of Physics Publishing,c2021 |
| International Standard Book Number | 9780750320818 |
| 797 2# - LOCAL ADDED ENTRY--CORPORATE NAME (RLIN) | |
| Corporate name or jurisdiction name as entry element | ProQuest (Firm) |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Biophysical Society-IOP Series |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://ebookcentral.proquest.com/lib/orpp/detail.action?docID=31253159">https://ebookcentral.proquest.com/lib/orpp/detail.action?docID=31253159</a> |
| Public note | Click to View |
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