Methods in Biotechnology. (Record no. 33179)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 11125nam a22005053i 4500 |
| 001 - CONTROL NUMBER | |
| control field | EBC7104461 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | MiAaPQ |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20240724115716.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d | |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr cnu|||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 240724s2016 xx o ||||0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9781119156802 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 9781119156789 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (MiAaPQ)EBC7104461 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (Au-PeEL)EBL7104461 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)1347023264 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | MiAaPQ |
| Language of cataloging | eng |
| Description conventions | rda |
| -- | pn |
| Transcribing agency | MiAaPQ |
| Modifying agency | MiAaPQ |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | TP248.24 .H664 2017 |
| 082 0# - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 660.6 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hong, Seung-Beom. |
| 245 10 - TITLE STATEMENT | |
| Title | Methods in Biotechnology. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | 1st ed. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Newark : |
| Name of producer, publisher, distributor, manufacturer | John Wiley & Sons, Incorporated, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2016. |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | ©2017. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (731 pages) |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 490 1# - SERIES STATEMENT | |
| Series statement | New York Academy of Sciences Series |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Intro -- Title Page -- Copyright -- Table of Contents -- Preface -- Acknowledgements -- Introduction to biotechnology lab -- Lab safety -- Good work habits -- Lab etiquette -- Lab record -- Lab report and assignment -- Further reading -- Laboratory Schedule (Methods in Biotechnology (MB)) -- Laboratory Schedule (Advanced Methods in Biotechnology (AMB) 1) -- Laboratory Schedule (AMB 2) -- About the companion website -- Part I: Methods in Biotechnology (MB) Laboratory Exercises -- Chapter 1: MB experiment 1: Lab measurements -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Pipetting skill -- Part II. Density and specific gravity -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 2: MB experiment 2: Use of the spectrophotometer and Beer's law -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 3: MB experiment 3: Making solutions and buffer efficacy -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. pH meter calibration -- Part II. Making solutions (to share four groups) -- Part III. Buffering capacity (addition of strong acid and strong base to buffered and unbuffered solutions) -- Part IV. Dilution effect on buffer pH and buffer capacity -- Discussion -- Post-lab assignment -- Further reading -- Chapter 4: MB experiment 4: Acid-base titration -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Two-point calibration of pH Meter -- Part II. Titration of acetic acid with NaOH -- Part III. Titration of HCl with NaOH -- Part IV. Data integration -- Discussion -- Post-lab assignment -- Further reading -- Chapter 5: MB experiment 5: Protein denaturation and precipitation -- Introduction -- Pre-lab assignment. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Materials and equipment -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 6: MB experiment 6: Bacterial transformation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Procedure (day 3) -- Discussion -- Post-lab assignment -- Further reading -- Chapter 7: MB experiment 7: GFP purification -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Discussion -- Further reading -- Chapter 8: MB experiment 8: SDS-PAGE analysis -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Discussion -- Post-lab assignment -- Further reading -- Chapter 9: MB experiment 9: DNA isolation -- Part I. Plasmid DNA isolation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Plasmid DNA isolation -- Part II. Genomic DNA isolation -- Introduction -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 10: MB experiment 10: PCR-based Alu-human DNA typing -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part E. Viewing and photography -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 11: MB experiment 11: Restriction enzyme digestion -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Chapter 12: MB experiment 12: Agarose gel electrophoresis -- Introduction -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 13: MB experiment 13: Ouchterlony and ELISA immunoassays -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Ouchterlony (double diffusion) assay -- Part II. ELISA assay -- Result -- Discussion -- Post-lab assignment -- Further reading. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Chapter 14: MB experiment 14: Testing plant substances for antimicrobial activity -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Result -- Discussion -- Post-lab assignment -- Further reading -- Chapter 15: MB experiment 15: Peroxidase enzyme activity assay -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Results -- Discussion -- Post-lab assignment -- References -- Part II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises -- chapter 16: AMB 1 experiment 16: Aseptic technique and culture handling -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- chapter 17: AMB 1 experiment 17: Yeast culture media preparation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- chapter 18: AMB 1 experiment 18: Growth curve -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 19: AMB 1 experiment 19: Mini plasmid prep -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (adapted from QIAprep miniprep kit) -- Post-lab assignment -- Additional information -- References -- Further reading -- Chapter 20: AMB 1 experiment 20: Restriction digestion, purification, concentration, and quantification of DNA -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 21: AMB 1 experiment 21: Polymerase chain reaction (PCR) -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Additional background information -- Further reading -- Chapter 22: AMB 1 experiment 22: TA, blunt end, SLIC, and CPEC cloning of PCR product -- Introduction. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Post-lab assignment -- Further reading -- Chapter 23: AMB 1 experiment 23: One-step multifragment assembly cloning -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Procedure (day 3) -- J. Screening of transformants -- Post-lab assignment -- References -- Further reading -- Chapter 24: AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Additional background information -- References -- Further reading -- chapter 25: AMB 1 experiment 25: Southern blot transfer -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (day 1) -- Procedure (day 2) -- Post-lab assignment -- Reference -- Further reading -- Chapter 26: AMB 1 experiment 26: Probe labeling and purification -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 27: AMB 1 experiment 27: Dot blot assay -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 28: AMB 1 experiment 28: Pre-hybridization, hybridization, and detection -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 29: AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure (adapted from SV total isolation kit, Promega and verso one-step RT PCR Kit, Life technologies) -- Post-lab assignment -- References -- Further reading -- Chapter 30: AMB 1 experiment 30: Yeast-based in vivo recombination cloning -- Introduction. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Isolation of overlapping DNA fragments (day 1) -- Part II. Yeast transformation (day 2) -- Further reading -- Chapter 31: AMB 1 experiment 31: Plasmid DNA isolation from yeast -- Introduction -- Materials and equipment -- Procedure -- References -- Chapter 32: AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA -- Introduction -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 33: AMB 1 experiment 33: X-gal filter lift assay -- Introduction -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 34: AMB 1 experiment 34: Protein quantitation assay -- Introduction -- Part I. Bradford assay -- Materials and equipment -- Procedure -- Part II. Lowry assay -- Materials and equipment -- Procedure -- Part III. BCA assay -- Material and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 35: AMB 1 experiment 35: Quantitative β-Galactosidase assay in yeast -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Part I. Permeabilized cell assay -- Part II. Cell-free protein extract assay -- Post-lab assignment -- Reference -- Further reading -- Chapter 36: AMB 1 experiment 36: Gel filtration chromatography (GFC) -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Chapter 37: AMB 1 experiment 37: Ion exchange chromatography (IEC) -- Background -- Pre-lab assignment -- Materials and equipment -- Procedure -- Post-lab assignment -- Further reading -- Part III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises -- Chapter 38: AMB 2 experiment 38: E. coli culture media preparation -- Introduction -- Pre-lab assignment -- Materials and equipment -- Procedure. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Post-lab assignment. |
| 588 ## - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Description based on publisher supplied metadata and other sources. |
| 590 ## - LOCAL NOTE (RLIN) | |
| Local note | Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2024. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology-Laboratory manuals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biotechnology-Technique. |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Main entry heading | Hong, Seung-Beom |
| Title | Methods in Biotechnology |
| Place, publisher, and date of publication | Newark : John Wiley & Sons, Incorporated,c2016 |
| International Standard Book Number | 9781119156789 |
| 797 2# - LOCAL ADDED ENTRY--CORPORATE NAME (RLIN) | |
| Corporate name or jurisdiction name as entry element | ProQuest (Firm) |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | New York Academy of Sciences Series |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://ebookcentral.proquest.com/lib/orpp/detail.action?docID=7104461">https://ebookcentral.proquest.com/lib/orpp/detail.action?docID=7104461</a> |
| Public note | Click to View |
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